Swarming motility, secretion of type 3 effectors and biofilm formation phenotypes exhibited within a large cohort of Pseudomonas aeruginosa clinical isolates.
Swarming motility, secretion of type 3 effectors and biofilm formation phenotypes exhibited within a large cohort of Pseudomonas aeruginosa clinical isolates.
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DOI：
10.1099/jmm.0.017715-0
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发表时间：
2010-05
影响因子：
3
通讯作者：
Kazmierczak BI
中科院分区：
文献类型：
--
作者：
Murray TS;Ledizet M;Kazmierczak BI
铜绿假单胞菌是一种革兰氏阴性机会致病菌，能够急性感染或持续定植敏感宿主。铜绿假单胞菌通过生物膜形成或群集运动在体外表面定植。行为的选择受到表面的物理性质和特定营养物质可用性的影响，并且受到也控制2型和3型蛋白质分泌的调节网络的影响。临床分离株的生物膜形成已得到充分研究。然而，人类分离株的群集行为尚未得到广泛的分析。我们从237名无囊性纤维化的住院患者中收集了分离株，并分析了每个分离株的运动性和分泌表型。我们发现生物膜形成和群集是负相关的，而群集与蛋白酶和3型外切酶的分泌呈正相关。大多数分离株能够3型分泌和生物膜形成，即使这些特征被认为有利于不同的发病机制。我们的数据表明，虽然临床分离株显示出不同的运动性，生物膜和分泌表型，但在实验室菌株中观察到的群集运动性和其他表型之间的许多预测关系也适用于从人类患者中分离的细菌。
Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen capable of acutely infecting or persistently colonizing susceptible hosts. P. aeruginosa colonizes surfaces in vitro by either biofilm formation or swarming motility. The choice of behavior is influenced by the physical properties of the surface and specific nutrient availability, and subject to regulatory networks that also govern Type 2 and Type 3 protein secretion. Biofilm formation by clinical isolates has been well-studied. However, the swarming behavior of human isolates has not been extensively analyzed. We collected isolates from 237 hospitalized patients without cystic fibrosis and analyzed motility and secretion phenotypes of each isolate. We found biofilm formation and swarming to be negatively associated, while swarming was positively associated with the secretion of both proteases and Type 3 exoenzymes. Most isolates were capable of Type 3 secretion and biofilm formation, even though these traits are considered to favor distinct modes of pathogenesis. Our data demonstrate that while clinical isolates display diverse motility, biofilm, and secretion phenotypes, many of the predicted relationships between swarming motility and other phenotypes observed in laboratory strains also hold true for bacteria isolated from human patients.
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影响因子：
--
作者：
Fonseca, Antonio Pedro;Correia, Petra;Tenreiro, Rogerio
通讯作者：
Tenreiro, Rogerio
影响因子：
3.6
作者：
Parkins, MD;Ceri, H;Storey, DG
通讯作者：
Storey, DG
影响因子：
3.6
作者：
Boles, BR;Thoendel, M;Singh, PK
通讯作者：
Singh, PK
DOI：
10.1073/pnas.060030097
发表时间：
2000-04-25
影响因子：
11.1
作者：
Rashid, MH;Kornberg, A
通讯作者：
Kornberg, A
影响因子：
5.7
作者：
Li, Li;Ledizet, Michel;Kazmierczak, Barbara I
通讯作者：
Kazmierczak, Barbara I