Granulocyte colony-stimulating factor (G-CSF) mediates bone resorption in periodontitis.
Granulocyte colony-stimulating factor (G-CSF) mediates bone resorption in periodontitis.
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粒细胞集落刺激因子（G-CSF）介导牙周炎中的骨吸收
DOI：
10.1186/s12903-021-01658-1
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发表时间：
2021-06-12
期刊：
影响因子：
2.9
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Yu H;Zhang T;Lu H;Ma Q;Zhao D;Sun J;Wang Z
研究背景粒细胞集落刺激因子（G-CSF）是一种重要的免疫因子，通过调节破骨细胞和成骨细胞的功能来调节骨代谢。骨质流失是牙周炎的一个严重和渐进的结果。然而，G-CSF对牙周炎症的作用机制尚未完全阐明。在这里，我们研究了抗G-CSF抗体是否可以抑制骨吸收的实验性牙周炎的模型，并研究了局部表达的G-CSF在牙周tissues.MethodsExperimental牙周炎诱导小鼠使用结扎。检测血清和骨髓中G-CSF的水平，并采用免疫荧光法分析G-CSF在牙周组织中的定位和表达。通过尾静脉注射给予牙周炎小鼠抗G-CSF抗体以评估骨吸收的抑制。通过显微计算机断层扫描分析进行三维重建以测量骨破坏相关参数。免疫荧光染色观察骨钙素阳性成骨细胞的存在;抗酒石酸酸性磷酸酶（TRAP）染色观察牙槽骨中破骨细胞的活性。免疫荧光分析显示，G-CSF主要表达于牙龈上皮细胞的细胞膜上，牙周炎组表达增强。此外，全身给予抗G-CSF抗体显著抑制牙槽骨吸收，如骨体积/总体积、骨表面积/骨体积、骨小梁厚度、骨小梁间距和骨小梁模式因子值的改善所证明的。免疫荧光分析显示骨钙素阳性成骨细胞的数量增加，而TRAP染色显示破骨细胞activity.ConclusionsG-CSF的表达水平显着上调血清和牙龈上皮细胞减少。同时，抗G-CSF抗体的施用可加速牙槽骨吸收，表明G-CSF可能是介导牙周炎骨丢失的重要免疫因子之一。
BackgroundGranulocyte colony-stimulating factor (G-CSF) is an important immune factor that mediates bone metabolism by regulating the functions of osteoclasts and osteoblasts. Bone loss is a serious and progressive result of periodontitis. However, the mechanisms underlying the effects of G-CSF on periodontal inflammation have yet not been completely elucidated. Here, we examined whether an anti-G-CSF antibody could inhibit bone resorption in a model of experimental periodontitis and investigated the local expression of G-CSF in periodontal tissues.MethodsExperimental periodontitis was induced in mice using ligatures. The levels of G-CSF in serum and bone marrow were measured; immunofluorescence was then performed to analyze the localization and expression of G-CSF in periodontal tissues. Mice with periodontitis were administered anti-G-CSF antibody by tail vein injection to assess the inhibition of bone resorption. Three-dimensional reconstruction was performed to measure bone destruction‐related parameters via micro-computed tomography analysis. Immunofluorescence staining was used to investigate the presence of osteocalcin-positive osteoblasts; tartrate-resistant acid phosphatase (TRAP) staining was used to observe osteoclast activity in alveolar bone.ResultsThe level of G-CSF in serum was significantly elevated in mice with periodontitis. Immunofluorescence analyses showed that G-CSF was mostly expressed in the cell membrane of gingival epithelial cells; this expression was enhanced in the periodontitis group. Additionally, systemic administration of anti-G-CSF antibody significantly inhibited alveolar bone resorption, as evidenced by improvements in bone volume/total volume, bone surface area/bone volume, trabecular thickness, trabecular spacing, and trabecular pattern factor values. Immunofluorescence analysis revealed an enhanced number of osteocalcin-positive osteoblasts, while TRAP staining revealed reduction of osteoclast activity.ConclusionsG-CSF expression levels were significantly up-regulated in the serum and gingival epithelial cells. Together, anti-G-CSF antibody administration could alleviates alveolar bone resorption, suggesting that G-CSF may be one of the essential immune factors that mediate the bone loss in periodontitis.
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影响因子：
4.3
作者：
Li, Sidan;Li, Tianshou;Qiu, Lugui
通讯作者：
Qiu, Lugui
影响因子：
6.7
作者：
Meyle, Joerg;Dommisch, Henrik;Herzberg, Mark
通讯作者：
Herzberg, Mark
影响因子：
6
作者：
Parkos, Charles A.
通讯作者：
Parkos, Charles A.
影响因子：
32.4
作者：
Semerad, CL;Liu, FL;Link, DC
通讯作者：
Link, DC
DOI：
10.3760/cma.j.issn.0253-2727.2015.05.014
发表时间：
2015-05
影响因子：
--
作者：
Li T;Li Q;Li S;Nie Y;Qiu L
通讯作者：
Qiu L