Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni.
Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni.
复制标题
Mansoni血吸虫中性接触引起的基因表达的微阵列分析。
DOI：
10.1186/1471-2164-8-181
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发表时间：
2007-06-20
期刊：
影响因子：
4.4
通讯作者：
El-Sayed, Najib M.
中科院分区：
文献类型：
--
作者：
Waisberg, Michael;Lobo, Francisco P.;Cerqueira, Gustavo C.;Passos, Liana K. J.;Carvalho, Omar S.;Franco, Gloria R.;El-Sayed, Najib M.
寄生吸虫曼氏血吸虫（Schistosoma mansoni）是血吸虫病的主要病原体之一，血吸虫病影响大约2亿人，主要是在发展中国家。由于许多病理学与雌虫产卵有关，因此了解卵子发生和性成熟的机制是发现有效药物治疗新靶点的重要一步。已知成虫雌虫仅在雄虫存在的情况下才完全发育，并且通过交配显著增加产卵和卵成熟的速率。为了研究与性成熟和产卵相关的基因转录本，我们利用DNA微阵列技术比较了性成熟和未成熟寄生虫的基因表达谱。对于每个实验，分析三个扩增的RNA微阵列杂交及其染料交换。我们的研究结果表明，265转录差异表达的成年女性和成年男性53成熟和未成熟的蠕虫进行比较时。在差异表达的基因中，55%在配对的雌性中以较高水平表达，而其余45%在未配对的中表达更多，56.6%在配对的雄性蠕虫中以较高水平表达，而其余43.4%在未成熟的寄生虫中表达更多。实时荧光定量RT-PCR分析验证了芯片结果。鉴定了几种新的成熟相关转录物。在单一性别雌性中上调的基因主要与能量产生相关（即碳水化合物和蛋白质代谢、前体代谢物和能量的产生、细胞catalysts以及细胞器组织和生物发生），而下调的基因与RNA代谢、活性氧代谢、电子传递、细胞器组织和生物发生以及蛋白质生物合成相关。我们的研究结果证实了以前的观察有关的基因表达诱导性成熟的女性蠕虫。他们还增加了S的列表。mansoni成熟相关的转录物相当大，因此打开了新的和令人兴奋的途径，为研究的配偶生物学和开发新的药物，针对染色体。
The parasitic trematode Schistosoma mansoni is one of the major causative agents of Schistosomiasis, a disease that affects approximately 200 million people, mostly in developing countries. Since much of the pathology is associated with eggs laid by the female worm, understanding the mechanisms involved in oogenesis and sexual maturation is an important step towards the discovery of new targets for effective drug therapy. It is known that the adult female worm only develops fully in the presence of a male worm and that the rates of oviposition and maturation of eggs are significantly increased by mating. In order to study gene transcripts associated with sexual maturation and oviposition, we compared the gene expression profiles of sexually mature and immature parasites using DNA microarrays. For each experiment, three amplified RNA microarray hybridizations and their dye swaps were analyzed. Our results show that 265 transcripts are differentially expressed in adult females and 53 in adult males when mature and immature worms are compared. Of the genes differentially expressed, 55% are expressed at higher levels in paired females while the remaining 45% are more expressed in unpaired ones and 56.6% are expressed at higher levels in paired male worms while the remaining 43.4% are more expressed in immature parasites. Real-time RT-PCR analysis validated the microarray results. Several new maturation associated transcripts were identified. Genes that were up-regulated in single-sex females were mostly related to energy generation (i.e. carbohydrate and protein metabolism, generation of precursor metabolites and energy, cellular catabolism, and organelle organization and biogenesis) while genes that were down-regulated related to RNA metabolism, reactive oxygen species metabolism, electron transport, organelle organization and biogenesis and protein biosynthesis. Our results confirm previous observations related to gene expression induced by sexual maturation in female schistosome worms. They also increase the list of S. mansoni maturation associated transcripts considerably, therefore opening new and exciting avenues for the study of the conjugal biology and development of new drugs against schistosomes.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
2.4
作者：
Gobert, GN;Stenzel, DJ;McManus, DP
通讯作者：
McManus, DP
影响因子：
2.1
作者：
Gobert, Geoffrey N.;McInnes, Russell;McManus, Donald P.
通讯作者：
McManus, Donald P.
DOI：
10.4269/ajtmh.1985.34.508
发表时间：
1985-01-01
影响因子：
3.3
作者：
ARONSTEIN, WS;STRAND, M
通讯作者：
STRAND, M
影响因子：
1.5
作者：
Fitzpatrick, JM;Johansen, MV;Hoffmann, KF
通讯作者：
Hoffmann, KF