A complete DNA sequence map of the ovine major histocompatibility complex.
A complete DNA sequence map of the ovine major histocompatibility complex.
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绵羊主要组织相容性复合体的完整 DNA 序列图。
DOI：
10.1186/1471-2164-11-466
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发表时间：
2010-08-10
期刊：
影响因子：
4.4
通讯作者：
Ma RZ
中科院分区：
文献类型：
--
作者：
Gao J;Liu K;Liu H;Blair HT;Li G;Chen C;Tan P;Ma RZ
绵羊主要组织相容性复合体（MHC）具有参与动物对感染性病原体的总体抗性/易感性的基因簇。然而，只有有限数量的绵羊MHC基因已被确定，没有足够的序列信息是可用的，相比，猪和牛。我们先前构建了一个基于BAC克隆的物理图谱，覆盖了绵羊MHC的整个I类、II类和III类区域。在这里，我们描述了一个完整的DNA序列图的组装绵羊MHC鸟枪测序的26个重叠BAC克隆。DNA鸟枪测序产生了大约8倍的基因组等效数据，成功地组装成一个完整的绵羊MHC序列图。该序列图跨越大约2，434，000个核苷酸的长度，几乎涵盖了目前已知的绵羊和牛的所有MHC基因座。基因注释鉴定了177个蛋白质编码基因/ORF，其中145个以前在绵羊中未报道，10个是绵羊种特异性的，在牛或其他哺乳动物中不存在。人、羊和牛之间的比较序列分析显示，除了II类外，MHC结构和位点顺序具有高度保守性，II类在羊和牛中被分成IIa和IIb亚区，由约18.5 Mb的大块非MHC常染色体分隔。此外，在绵羊MHC区域中首次预测了总共18个非蛋白编码microRNA。利用鸟枪法对26个重叠BAC克隆进行测序，成功构建了绵羊MHC DNA序列图。这使得绵羊成为继牛之后第二个完整的MHC序列信息可用于进化和功能研究的反刍动物物种。比较分析的结果支持这样一个假设，即含有MHC的祖先染色体的倒位已经形成了反刍动物的MHC结构，正如我们目前在绵羊和牛中观察到的那样。绵羊MHC区域中相对大量的microRNA的鉴定有助于提供microRNA积极参与MHC基因表达和功能调节的证据。
The ovine Major Histocompatibility Complex (MHC) harbors clusters of genes involved in overall resistance/susceptibility of an animal to infectious pathogens. However, only a limited number of ovine MHC genes have been identified and no adequate sequence information is available, as compared to those of swine and bovine. We previously constructed a BAC clone-based physical map that covers entire class I, class II and class III region of ovine MHC. Here we describe the assembling of a complete DNA sequence map for the ovine MHC by shotgun sequencing of 26 overlapping BAC clones. DNA shotgun sequencing generated approximately 8-fold genome equivalent data that were successfully assembled into a finished sequence map of the ovine MHC. The sequence map spans approximately 2,434,000 nucleotides in length, covering almost all of the MHC loci currently known in the sheep and cattle. Gene annotation resulted in the identification of 177 protein-coding genes/ORFs, among which 145 were not previously reported in the sheep, and 10 were ovine species specific, absent in cattle or other mammals. A comparative sequence analyses among human, sheep and cattle revealed a high conservation in the MHC structure and loci order except for the class II, which were divided into IIa and IIb subregions in the sheep and cattle, separated by a large piece of non-MHC autosome of approximately 18.5 Mb. In addition, a total of 18 non-protein-coding microRNAs were predicted in the ovine MHC region for the first time. An ovine MHC DNA sequence map was successfully assembled by shotgun sequencing of 26 overlapping BAC clone. This makes the sheep the second ruminant species for which the complete MHC sequence information is available for evolution and functional studies, following that of the bovine. The results of the comparative analysis support a hypothesis that an inversion of the ancestral chromosome containing the MHC has shaped the MHC structures of ruminants, as we currently observed in the sheep and cattle. Identification of relative large numbers of microRNAs in the ovine MHC region helps to provide evidence that microRNAs are actively involved in the regulation of MHC gene expression and function.
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影响因子：
4.4
作者：
Glass, EJ;Oliver, RA;Russell, GC
通讯作者：
Russell, GC
影响因子：
3.2
作者：
Herrmann-Hoesing, Lynn M.;White, Stephen N.;Knowles, Donald P.
通讯作者：
Knowles, Donald P.
影响因子：
3.2
作者：
Bonneaud, C;Richard, M;Sorci, G
通讯作者：
Sorci, G
影响因子：
9.8
作者：
Belov K;Deakin JE;Papenfuss AT;Baker ML;Melman SD;Siddle HV;Gouin N;Goode DL;Sargeant TJ;Robinson MD;Wakefield MJ;Mahony S;Cross JG;Benos PV;Samollow PB;Speed TP;Graves JA;Miller RD
通讯作者：
Miller RD
影响因子：
4.4
作者：
Itoh, T;Watanabe, T;Takasuga, A
通讯作者：
Takasuga, A