Analysis of cerebrospinal fluid and cerebrospinal fluid cells from patients with multiple sclerosis for detection of JC virus DNA.
Analysis of cerebrospinal fluid and cerebrospinal fluid cells from patients with multiple sclerosis for detection of JC virus DNA.
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DOI：
10.1177/1352458508096870
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发表时间：
2009-01
期刊：
影响因子：
--
通讯作者：
Major E
中科院分区：
文献类型：
--
作者：
Iacobaeus E;Ryschkewitsch C;Gravell M;Khademi M;Wallstrom E;Olsson T;Brundin L;Major E
1) 与对照组相比，确定多发性硬化症 (MS) 患者的脑脊液 (CSF) 和血液中是否存在 JC 病毒 (JCV) DNA；2) 根据 JCV DNA 的存在，查明我们的临床材料是否包括任何有进展性多灶性白质脑病 (PML) 风险的患者。对 217 名 MS 患者、86 名临床孤立综合征 (CIS) 患者和 212 名其他神经系统疾病 (OND) 患者的脑脊液和血浆中 JCV DNA 的患病率进行了分析。此外，我们还分析了脑脊液细胞（首次报告单个样本中脑脊液细胞中存在 JCV DNA）以及 MS (n = 49)、CIS (n = 14) 和 OND (n = 53) 亚组中的外周血细胞。在一份 MS 无细胞 CSF 样品和一份 MS CSF 细胞样品中检测到低拷贝数的 JCV DNA。这些人都没有任何 PML 迹象，也没有在随访期间患上这种疾病。此外，两个 OND 血浆样本呈 JCV DNA 阳性，而所有其他样本均未检测到病毒。在多发性硬化症和其他疾病中偶尔会观察到低拷贝数的 JCV DNA，这可能是人类 JC 病毒正常生物学的一部分。这项研究不支持 MS 患者发生 PML 的风险增加的假设，因此将 CSF 筛查作为 PML 的可测量风险是没有用的。
1) To determine whether JC virus (JCV) DNA was present in the cerebrospinal fluid (CSF) and blood from patients with multiple sclerosis (MS) in comparison with controls and 2) to find out if our clinical material, based on presence of JCV DNA, included any patient at risk for progressive multifocal leukoencephalopathy (PML). The prevalence of JCV DNA was analyzed in CSF and plasma from 217 patients with MS, 86 patients with clinically isolated syndrome (CIS), and 212 patients with other neurological diseases (OND). In addition, we analyzed CSF cells, the first report of JCV DNA in CSF cells in a single sample, and peripheral blood cells in a subgroup of MS (n = 49), CIS (n = 14) and OND (n = 53). A low copy number of JCV DNA was detected in one MS cell free CSF sample and in one MS CSF cell samples. None of these had any signs of PML or developed this disease during follow-up. In addition, two OND plasma samples were JCV DNA positive, whereas all the other samples had no detectable virus. A low copy number of JCV DNA may occasionally be observed both in MS and other diseases and may occur as part of the normal biology of JC virus in humans. This study does not support the hypothesis that patients with MS would be at increased risk to develop PML, and consequently screening of CSF as a measurable risk for PML is not useful.
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影响因子：
5.4
作者：
Monaco, MCG;Atwood, WJ;Major, EO
通讯作者：
Major, EO
影响因子：
3.2
作者：
Dörries, K;Sbiera, S;Dörries, R
通讯作者：
Dörries, R
影响因子：
5.1
作者：
Alvarez-Lafuente, R.;Garcia-Montojo, M.;Arroyo, R.
通讯作者：
Arroyo, R.
影响因子：
5.8
作者：
Ferrante, P;Omodeo-Zorini, E;Caputo, D
通讯作者：
Caputo, D
影响因子：
12.7
作者：
Randhawa, P;Uhrmacher, J;Weck, K
通讯作者：
Weck, K