The thermogenic actions of natriuretic peptide in brown adipocytes: The direct measurement of the intracellular temperature using a fluorescent thermoprobe.
The thermogenic actions of natriuretic peptide in brown adipocytes: The direct measurement of the intracellular temperature using a fluorescent thermoprobe.
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DOI：
10.1038/s41598-017-13563-1
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发表时间：
2017-10-11
影响因子：
4.6
通讯作者：
Yoshimura M
中科院分区：
文献类型：
--
作者：
Kimura H;Nagoshi T;Yoshii A;Kashiwagi Y;Tanaka Y;Ito K;Yoshino T;Tanaka TD;Yoshimura M
除了利钠肽（NPs）对心血管系统的各种作用外，NPs诱导脂肪组织布朗宁和激活产热程序的可能性也越来越受到人们的关注。我们建立了一个直接的细胞内温度测量系统，并使用荧光温度探针研究了A型NP（ANP）对棕色脂肪细胞的产热效应。将该探针成功地导入大鼠棕色脂肪细胞，并在荧光显微镜下测量了其荧光强度比值随温度的变化。在与ANP孵育1小时后，与未处理的对照相比，ANP处理的脂肪细胞中荧光强度比率的变化程度显著更高（P < 0.01）。ANP处理增加解偶联蛋白-1（UCP 1）mRNA水平，这是脂肪细胞产热的标志之一，而细胞内ATP含量没有变化，表明线粒体解偶联呼吸。有趣的是，当棕色脂肪细胞在35 °C下孵育时，ANP的这些产热作用比在37 °C下更突出。此外，p38 MAPK抑制可抵消ANP诱导的细胞内温度升高和UCP 1表达。总之，本研究通过使用一种新的细胞内温度测量方法直接证明了ANP在棕色脂肪细胞中的产热作用。
In addition to the various effects of natriuretic peptides (NPs) on cardiovascular systems, increasing attention is being paid to the possibility that NPs induce adipose tissue browning and activate thermogenic program. We herein established a direct intracellular temperature measurement system using a fluorescent thermoprobe and investigated the thermogenic effects of A-type NP (ANP) on brown adipocytes. The thermoprobe was successfully introduced into rat brown adipocytes, and the temperature dependent change in fluorescence intensity ratio was measured using a fluorescence microscope. After one-hour incubation with ANP, the degree of the change in fluorescence intensity ratio was significantly higher in ANP-treated (P < 0.01) adipocytes compared to untreated controls. The ANP treatment increased uncoupling protein-1 (UCP1) mRNA levels, which is one of the markers of thermogenesis in adipocytes, while the intracellular ATP content was not changed, indicating mitochondrial uncoupled respiration. Intriguingly, these thermogenic actions of ANP were more prominent when brown adipocytes were incubated at 35 °C than at 37 °C. Moreover, the increase in the intracellular temperature and the expression of UCP1 induced by ANP were cancelled by p38MAPK inhibition. Taken together, this study directly demonstrated the thermogenic actions of ANP in brown adipocytes through the use of a novel method of intracellular temperature measurement.
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影响因子：
16.6
作者：
Hattori K;Naguro I;Okabe K;Funatsu T;Furutani S;Takeda K;Ichijo H
通讯作者：
Ichijo H
影响因子：
3.7
作者：
Minai K;Ogawa T;Kawai M;Komukai K;Tanaka T;Ogawa K;Nagoshi T;Arase S;Morimoto S;Inoue Y;Sekiyama H;Urabe A;Matsuo S;Hongo K;Yoshimura M
通讯作者：
Yoshimura M
影响因子：
7.7
作者：
Miyashita K;Itoh H;Tsujimoto H;Tamura N;Fukunaga Y;Sone M;Yamahara K;Taura D;Inuzuka M;Sonoyama T;Nakao K
通讯作者：
Nakao K
DOI：
10.1016/j.tem.2011.07.003
发表时间：
2011-11
期刊：
Trends in endocrinology and metabolism: TEM
影响因子：
--
作者：
Iacobellis G;Bianco AC
通讯作者：
Bianco AC
影响因子：
15.9
作者：
Nagoshi, T;Matsui, T;Rosenzweig, A
通讯作者：
Rosenzweig, A