Amyloid A gene family expression in different mouse tissues.
Amyloid A gene family expression in different mouse tissues.
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DOI：
10.1084/jem.164.6.2006
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发表时间：
1986-12-01
期刊：
影响因子：
--
通讯作者：
Benditt EP
中科院分区：
文献类型：
--
作者：
Meek RL;Benditt EP
血清淀粉样蛋白 A (SAA) 是高密度脂蛋白 (HDL) 的主要急性期反应物和脱辅基蛋白。 SAA 由三个活性基因家族编码。我们检查了肝脏表达，并寻找注射酪蛋白或 LPS 后三种 SAA mRNA 的肝外表达。使用 SAA cDNA 进行的研究（可检测所有三种 SAA mRNA）显示，注射酪蛋白后，肝脏 SAA mRNA 升高了约 1,000 倍。肾上腺以低水平表达SAA mRNA（肝脏水平的0.5%），并且是注射酪蛋白后SAA mRNA升高的唯一肝外组织。未受刺激的对照动物的小肠（主要是回肠）和大肠的 SAA mRNA 水平比对照肝脏高 5 倍和 15 倍。 LPS 还将肝脏 SAA mRNA 升高约 1,000 倍。然而，与注射酪蛋白相反，检查的每个肝外组织均表达 SAA mRNA。肺和肾含有 2-5%，大肠含有近 10% 的 SAA mRNA 水平，存在于肝脏 RNA 中。其余组织中的 SAA mRNA 水平较低，范围从脑和胰腺中的 0.1% 到小肠中的 1.0%，其中回肠的含量是十二指肠的 50 倍。用 SAA1、SAA2 和 SAA3 mRNA 特异性寡核苷酸探针对肝脏进行分析表明，施用酪蛋白后，SAA1 和 SAA2 mRNA 比 SAA3 mRNA 升高约 50 倍。然而，LPS 同等地诱导所有三种 SAA mRNA。在肝外组织中，SAA1、SAA2和SAA3 mRNA表达差异，可分为三大类：表达所有三种基因的组织、表达SAA1和SAA3的组织以及主要或仅表达SAA3的组织。
Serum amyloid A (SAA) is a major acute-phase reactant and apoprotein of high density lipoprotein (HDL). SAA is encoded by a family of three active genes. We examined hepatic expression and searched for extrahepatic expression of the three SAA mRNAs after injection with casein or LPS. Studies using an SAA cDNA, which detects all three SAA mRNAs, revealed that after casein injection liver SAA mRNA was elevated approximately 1,000-fold. Adrenal gland expressed SAA mRNA at a low level (0.5% of hepatic level), and was the only extrahepatic tissue with elevated SAA mRNA after casein injection. The small intestine, primarily the ileum, and the large intestine of unstimulated control animals contained 5- and 15-fold higher SAA mRNA levels than control liver. LPS also elevated liver SAA mRNA approximately 1,000-fold. However, in contrast to casein injection, every extrahepatic tissue examined expressed SAA mRNA. Lung and kidney contained 2-5% and large intestine contained nearly 10% of SAA mRNA levels found in liver RNA. SAA mRNA levels were lower in the remaining tissues and ranged from 0.1% in the brain and pancreas to 1.0% in the small intestine, with the ileum containing 50-fold more than the duodenum. Analysis of liver with SAA1, SAA2, and SAA3 mRNA-specific oligonucleotide probes revealed that SAA1 and SAA2 mRNA were elevated approximately 50-fold higher than SAA3 mRNA after casein administration. LPS, however, induced all three SAA mRNAs equally. In extrahepatic tissues, SAA1, SAA2, and SAA3 mRNAs were expressed differentially and can be grouped into three general classes: tissues expressing all three genes, tissues expressing SAA1 and SAA3, and tissues expressing predominantly or only SAA3.
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影响因子：
2.9
作者：
LEHRACH, H;DIAMOND, D;BOEDTKER, H
通讯作者：
BOEDTKER, H
影响因子：
15.3
作者：
MEEK, RL;HOFFMAN, JS;BENDITT, EP
通讯作者：
BENDITT, EP
DOI：
10.1111/j.1749-6632.1982.tb22124.x
发表时间：
1982-01-01
影响因子：
5.2
作者：
KUSHNER, I
通讯作者：
KUSHNER, I
DOI：
10.1073/pnas.74.9.4025
发表时间：
1977-01-01
影响因子：
11.1
作者：
BENDITT, EP;ERIKSEN, N
通讯作者：
ERIKSEN, N
DOI：
10.1073/pnas.76.8.4092
发表时间：
1979-01-01
影响因子：
11.1
作者：
BENDITT, EP;ERIKSEN, N;HANSON, RH
通讯作者：
HANSON, RH