Potential antioxidant bioactive peptides from camel milk proteins.
Potential antioxidant bioactive peptides from camel milk proteins.
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DOI：
10.1016/j.aninu.2018.05.004
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发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Miyata T
中科院分区：
文献类型：
--
作者：
Ibrahim HR;Isono H;Miyata T
骆驼奶传统上被认为具有药用特性，它具有潜在的健康益处，可以帮助治疗多种疾病。特别是它最接近母乳，营养成分和生物活性成分含量高。本研究的目的是探索源自骆驼奶蛋白质组分的抗氧化肽。骆驼乳蛋白 (CMP) 被分离成骆驼酪蛋白 (CCP) 和骆驼乳清蛋白 (CWP)，用胃蛋白酶水解，分别产生胃消化物 P-CCP 和 P-CWP。 RP-HPLC 用于从 P-CCP 和 P-CWP 中分离肽。使用黄嘌呤氧化酶（XOD）的超氧阴离子生成系统和2,2-二苯基-1-三硝基苯肼（DPPH）清除测定法评估抗氧化活性。使用基质辅助激光解吸电离飞行时间质谱 (MALDI-TOF-MS) 分析活性肽，同时鉴定出许多质量范围为 913 至 2,951 Da 的抗氧化肽，主要源自 α-酪蛋白、乳铁蛋白和乳铁蛋白。当酵母细胞用作线粒体疾病建模系统时，酪蛋白和乳清组分中的肽显着增强了酵母细胞对过氧化物诱导的氧化应激的耐受性。结果表明，骆驼奶中的酪蛋白和乳清蛋白均具有具有显着自由基清除活性的生物活性肽，因此预示着它们作为营养保健品或治疗性肽用于预防和治疗氧化应激相关疾病的潜在机会。
Camel milk is traditionally considered to have medicinal characteristics that it has potential health benefits and could help to treat several illnesses. Particularly, it is closest to human breast milk and has high levels of nutrients and bioactive components. The aim of this study was to explore the antioxidant peptides derived from protein fractions of camel milk. Camel milk proteins (CMP) were fractionated into camel casein protein (CCP) and camel whey protein (CWP), which were hydrolyzed with pepsin to produce peptic digests P-CCP and P-CWP, respectively. RP-HPLC was used for fractionation of the peptides from the P-CCP and P-CWP. The antioxidant activities were evaluated using superoxide anion generating system of xanthine oxidase (XOD) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. Active peptides were analyzed using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) whereas a number of antioxidant peptides, with masses ranging from 913 to 2,951 Da, derived mainly from alpha-casein, lactophorin and lactoferrin, were identified. When yeast cells are used as a system for modeling mitochondrial disease, the peptides in caseins and whey fractions significantly enhanced the tolerance of yeast cells against peroxide-induced oxidative stress. The results show that both caseins and whey proteins of camel milk possess bioactive peptides with significant radical-scavenging activities and thus herald a fascinating opportunity for their potential as nutraceuticals or therapeutic peptides for prevention and treatment of oxidative stress-associated diseases.
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