Biologic activity of porphyromonas endodontalis complex lipids.
Biologic activity of porphyromonas endodontalis complex lipids.
复制标题
DOI：
10.1016/j.joen.2014.02.017
复制
发表时间：
2014-09
影响因子：
4.2
通讯作者：
Nichols FC
中科院分区：
文献类型：
--
作者：
Mirucki CS;Abedi M;Jiang J;Zhu Q;Wang YH;Safavi KE;Clark RB;Nichols FC
继发于牙髓坏死的根尖周感染与牙髓细菌污染有关。牙髓卟啉单胞菌是一种革兰氏阴性菌，被认为是牙髓的病原体。牙龈假单胞菌与牙髓假单胞菌具有亲缘关系，可合成多种具有生物活性的新型复合脂类，包括促进破骨细胞生成和激活破骨细胞。本研究的目的是提取和鉴定牙髓假单胞菌的组成脂类，并评价其在巨噬细胞系RAW 264.7中促进促炎分泌反应的能力以及促进破骨细胞生成和抑制成骨细胞活性的能力。用高效液相色谱对两种细菌的组成脂进行了分离，并用电喷雾-质谱仪(ESI-MS)或电喷雾-质谱仪(ESI-MS/MS)对其结构进行了表征，然后将其毒力潜力与已知的牙龈假单胞菌生物活性脂类进行了比较。牙髓假单胞菌总脂能促进RAW 264.7细胞分泌肿瘤坏死因子-α，而且丝氨酸脂似乎是这种作用的主要部分。牙髓假单胞菌脂质制剂也能促进RAW 264.7细胞破骨细胞的形成，但培养的成骨细胞分化受到抑制，而且似乎依赖于TLR2的表达。这些效应强调了牙髓杆菌脂类在促进炎症和骨细胞激活过程中的重要性，这些过程可能导致根尖周病理。
Periapical infections secondary to pulpal necrosis are associated with bacterial contamination of the pulp. Porphyromonas endodontalis, a Gram-negative organism, is considered to be a pulpal pathogen. P. gingivalis is phylogenetically related to P. endodontalis and synthesizes several classes of novel complex lipids that possess biological activity, including the capacity to promote osteoclastogenesis and osteoclast activation. The purpose of this study was to extract and characterize constituent lipids of P. endodontalis, and evaluate their capacity to promote pro-inflammatory secretory responses in the macrophage cell line, RAW 264.7, as well as their capacity to promote osteoclastogenesis and inhibit osteoblast activity. Constituent lipids of both organisms were fractionated by HPLC and were structurally characterized using electrospray-mass spectrometry (ESI-MS) or ESI-MS/MS. The virulence potential of P. endodontalis lipids was then compared with known biologically active lipids isolated from P. gingivalis. P. endodontalis total lipids were shown to promote TNF-α secretion from RAW 264.7 cells and the serine lipid fraction appeared to account for the majority of this effect. P. endodontalis lipid preparations also increased osteoclast formation from RAW 264.7 cells but osteoblast differentiation in culture was inhibited and appeared to be dependent on TLR2 expression. These effects underscore the importance of P. endodontalis lipids in promoting inflammatory and bone cell activation processes that could lead to periapical pathology.
登录
查看更多内容
影响因子：
6.5
作者：
Nichols, FC;Riep, B;Smith, MB
通讯作者：
Smith, MB
影响因子：
--
作者：
Sakamoto, M;Rôças, IN;Benno, Y
通讯作者：
Benno, Y
影响因子：
6.5
作者：
Nichols, FC;Riep, B;Smith, MB
通讯作者：
Smith, MB
牙髓卟啉单胞菌脂多糖通过小鼠成骨细胞诱导 RANKL，其方式与大肠杆菌脂多糖不同
影响因子：
4.2
作者：
Tang, Yin;Sun, Feifei;Zhou, Xuedong
通讯作者：
Zhou, Xuedong
DOI：
10.1006/bmmb.1994.1068
发表时间：
1994-12-01
期刊：
BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY
影响因子：
--
作者：
OGURA, N;SHIBATA, Y;ABIKO, Y
通讯作者：
ABIKO, Y