Gene Expression Associated with Early and Late Chronotypes in Drosophila melanogaster.
Gene Expression Associated with Early and Late Chronotypes in Drosophila melanogaster.
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DOI：
10.3389/fneur.2015.00100
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发表时间：
2015
影响因子：
3.4
通讯作者：
Tauber E
中科院分区：
文献类型：
--
作者：
Pegoraro M;Picot E;Hansen CN;Kyriacou CP;Rosato E;Tauber E
生物钟为节律性的行为和代谢功能提供了时间框架。在工业化、工作和社会压力的现代时代，生物钟的功能受到损害，并可能对健康产生不利和慢性影响。了解生物钟功能，特别是昼夜相位偏好（时辰型）的个体差异，以及这些时辰型背后的分子机制，可能会导致可以消除生物钟功能障碍并改善人类（和动物）健康和福利的干预措施。我们的初步研究表明，果蝇和人类一样，可以被归类为早起的“云雀”或晚起的“猫头鹰”，为这些类型的研究提供了一个方便的模型系统。我们已经从蛹的情况中鉴定出对早晨羽化（早或E）的偏好增加或对晚上羽化（晚或L）的偏好更明显的蝇株。我们在E和L品系中以4小时间隔在羽化前一天（蛹化后第四天）对蛹进行取样，并通过RNA-seq检查基因表达的差异。我们已经确定了E和L株之间的差异表达的转录本，这为随后的果蝇时间型及其人类直系同源物的研究提供了候选基因。
The circadian clock provides the temporal framework for rhythmic behavioral and metabolic functions. In the modern era of industrialization, work, and social pressures, clock function is jeopardized, and can result in adverse and chronic effects on health. Understanding circadian clock function, particularly individual variation in diurnal phase preference (chronotype), and the molecular mechanisms underlying such chronotypes may lead to interventions that could abrogate clock dysfunction and improve human (and animal) health and welfare. Our preliminary studies suggested that fruit-flies, like humans, can be classified as early rising “larks” or late rising “owls,” providing a convenient model system for these types of studies. We have identified strains of flies showing increased preference for morning emergence (Early or E) from the pupal case, or more pronounced preference for evening emergence (Late or L). We have sampled pupae the day before eclosion (fourth day after pupariation) at 4 h intervals in the E and L strains, and examined differences in gene expression by RNA-seq. We have identified differentially expressed transcripts between the E and L strains, which provide candidate genes for subsequent studies of Drosophila chronotypes and their human orthologs.
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