An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function

An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function
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小麦条锈菌的效应蛋白靶向叶绿体并抑制叶绿体功能

DOI:
10.1038/s41467-019-13487-6
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发表时间:
2019-12
影响因子:
16.6
通讯作者:
Xiaojie Wang
Xiaojie Wang
中科院分区:
综合性期刊1区
文献类型:
--
作者:
Qiang Xu;Chunlei Tang;Xiaodong Wang;Shutian Sun;Jinren Zhao;Zhensheng Kang;Xiaojie Wang

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叶绿体对于光合作用和植物对微生物病原体的免疫是重要的。在这里,我们确定了吸器特异性蛋白(Pst_12806)从小麦条锈菌,条锈菌。转座到叶绿体中并影响叶绿体功能的蛋白质。Pst_12806的瞬时表达抑制烟草植物中BAX诱导的细胞死亡并降低小麦中假单胞菌诱导的过敏反应Pst_12806通过减少胼胝质沉积和防御相关基因的表达来抑制植物的基础免疫。Pst_12806在感染过程中被上调,其敲低(通过宿主诱导的基因沉默)降低Pst的生长和发育,可能是由于增加ROS积累。Pst_12806与小麦TaISP蛋白(细胞色素b6-f复合物的推定组分)的C-末端Rieske结构域相互作用。Pst_12806在植物中的表达降低了电子传递速率、光合作用和叶绿体衍生的ROS的产生。在感病小麦品种中通过病毒诱导的基因沉默来沉默TaISP,从而减少真菌生长和夏孢子发育,表明对Pst感染的抗性增加。
Chloroplasts are important for photosynthesis and for plant immunity against microbial pathogens. Here we identify a haustorium-specific protein (Pst_12806) from the wheat stripe rust fungus,Puccinia striiformisf. sp.tritici(Pst), that is translocated into chloroplasts and affects chloroplast function. Transient expression ofPst_12806inhibits BAX-induced cell death in tobacco plants and reducesPseudomonas-induced hypersensitive response in wheat. It suppresses plant basal immunity by reducing callose deposition and the expression of defense-related genes.Pst_12806is upregulated during infection, and its knockdown (by host-induced gene silencing) reducesPstgrowth and development, likely due to increased ROS accumulation. Pst_12806 interacts with the C-terminal Rieske domain of the wheat TaISP protein (a putative component of the cytochrome b6-f complex). Expression ofPst_12806in plants reduces electron transport rate, photosynthesis, and production of chloroplast-derived ROS. SilencingTaISPby virus-induced gene silencing in a susceptible wheat cultivar reduces fungal growth and uredinium development, suggesting an increase in resistance againstPstinfection.
DOI: 10.1038/srep44598
发表时间: 2017-03-16
期刊: Scientific reports
影响因子: 4.6
作者:
Sperschneider J;Catanzariti AM;DeBoer K;Petre B;Gardiner DM;Singh KB;Dodds PN;Taylor JM
通讯作者: Taylor JM
DOI: 10.1007/bf00394782
发表时间: 1988-05
期刊: Planta
影响因子: 4.3
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DOI: 10.1073/pnas.151262398
发表时间: 2001-07
影响因子: 11.1
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DOI: 10.1186/1746-4811-10-21
发表时间: 2014
期刊: Plant methods
影响因子: 5.1
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DOI: 10.1073/pnas.96.14.7956
发表时间: 1999-07-06
影响因子: 11.1
作者:
Lacomme, C;Cruz, SS
通讯作者: Cruz, SS