Antifungal Effect of Piezoelectric Charges on PMMA Dentures.
Antifungal Effect of Piezoelectric Charges on PMMA Dentures.
复制标题
压电电荷对PMMA假牙的抗真菌作用。
DOI：
10.1021/acsbiomaterials.1c00926
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发表时间：
2021-10-11
影响因子：
5.8
通讯作者：
Orrego, Santiago
中科院分区：
文献类型：
--
作者：
Montoya, Carolina;Kurylec, Julia;Baraniya, Divyashri;Tripathi, Aparna;Puri, Sumant;Orrego, Santiago
关键词：
念珠菌相关假牙口腔炎是一种复发性疾病，影响高达 67% 的假牙佩戴者。由于其理想的物理、机械和美学特性，聚甲基丙烯酸甲酯 (PMMA) 仍然是制造假牙的主要材料。然而，其抗菌性能的提高仍然是一个挑战。为了满足这一需求，我们开发了填充钛酸钡 (BaTiO3) 压电纳米颗粒的 PMMA 复合材料，以达到治疗效果。在连续循环机械负载下，在复合材料表面培养白色念珠菌生物膜，以激活压电电荷并模拟咀嚼模式。通过测量生物膜生物量、代谢活性和活细胞数量来评估生物膜和生物材料之间的相互作用。为了探索抗真菌机制，使用逆转录聚合酶链反应评估了编码粘附素和超氧化物歧化酶的基因表达的变化。通过添加压电纳米粒子，我们观察到与标准 PMMA 相比，生物膜的形成和酵母菌向菌丝转变的干扰显着减少。此外，我们观察到，与静态/未变形表面相比，没有抗真菌剂的生物材料表面的循环变形产生了增加的生物量、代谢活性和大量活细胞。循环变形似乎是一种新的机械生物学信号，通过增加酵母菌丝过渡基因的表达，使白色念珠菌细胞具有致病性和毒力。这项研究的结果为抗真菌假牙的设计提供了新的机会，以改善临床服务并减少对清洁方法的需求。
Candida-associated denture stomatitis is a recurring disease affecting up to 67% of denture wearers. Poly(methyl methacrylate) (PMMA) remains the main material employed in the fabrication of dentures due to its desirable physical, mechanical, and aesthetic properties. However, the improvement of its antimicrobial properties remains a challenge. To address this need, we developed PMMA composite filled with piezoelectric nanoparticles of barium titanate (BaTiO3) for therapeutic effects. Candida albicans biofilms were cultivated on the surface of the composites under continuous cyclic mechanical loading to activate the piezoelectric charges and to resemble mastication patterns. The interactions between biofilms and biomaterials were evaluated by measuring the biofilm biomass, metabolic activity, and the number of viable cells. To explore the antifungal mechanisms, changes in the expression of genes encoding adhesins and superoxide dismutase were assessed using reverse transcription-polymerase chain reaction. With the addition of piezoelectric nanoparticles, we observed a significant reduction in the biofilm formation and interference in the yeast-to-hyphae transition compared to the standard PMMA. Moreover, we observed that the cyclic deformation of biomaterial surfaces without antifungal agents produced increased biomass, metabolic activity, and a number of viable cells compared to the static/no-deformed surfaces. Cyclic deformation appears to be a novel mechanobiological signal that enables pathogenicity and virulence of C. albicans cells with increased expression of the yeast-to-hyphae transition genes. The outcome of this study opens new opportunities for the design of antifungal dentures for improved clinical service and reduced need for cleaning methods.
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影响因子：
5
作者：
Lin, DM;Kalachandra, S;Offenbacher, S
通讯作者：
Offenbacher, S
DOI：
10.1111/jopr.13118
发表时间：
2019-11-26
影响因子：
4
作者：
AlBin-Ameer, Mohammed Ali;Alsrheed, Mahdi Y.;Gad, Mohammed M.
通讯作者：
Gad, Mohammed M.
DOI：
10.1016/j.jobcr.2020.04.012
发表时间：
2020-04-01
影响因子：
--
作者：
Ahmad, Nafis;Jafri, Zeba;Khan, Zishan H
通讯作者：
Khan, Zishan H
DOI：
10.3390/ma14010221
发表时间：
2021-01-05
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Meirowitz A;Rahmanov A;Shlomo E;Zelikman H;Dolev E;Sterer N
通讯作者：
Sterer N
影响因子：
3.3
作者：
Martchenko, M;Alarco, AM;Whiteway, M
通讯作者：
Whiteway, M