Effect of Denture Base Fabrication Technique on Candida albicans Adhesion In Vitro.

Effect of Denture Base Fabrication Technique on Candida albicans Adhesion In Vitro.
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DOI:
10.3390/ma14010221
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发表时间:
2021-01-05
期刊:
Materials (Basel, Switzerland)
影响因子:
--
通讯作者:
Sterer N
Sterer N
中科院分区:
其他
文献类型:
--
作者:
Meirowitz A;Rahmanov A;Shlomo E;Zelikman H;Dolev E;Sterer N

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戴义齿性口炎是口腔念珠菌病的一种常见表现,约65%的戴义齿者受到影响。这种情况是由白色念珠菌粘附到义齿基托丙烯酸树脂上引起的。本研究旨在测试传统和新型制作方法对义齿基托样品上白色念珠菌粘附的体外影响。使用:(i)计算机化研磨,(ii)3D打印,(iii)热固化,和(iv)冷固化来制造基于丙烯酸的盘。测试圆盘的表面粗糙度(Ra)、疏水性(接触角)、粘蛋白吸附(Bradford测定)和白色念珠菌粘附。与热固化相比,3D打印显著增加了微生物细胞的粘附力,而计算机铣削显著降低了粘附力。这些结果与粘蛋白吸附水平而不是表面粗糙度有关。结果表明,与传统的热固化义齿基托制造相比,3D打印可能会增加患义齿性口炎的风险,而计算机铣削可能会降低风险。
Denture stomatitis is a common manifestation of oral candidiasis affecting some 65% of denture wearers. This condition is initiated by the adherence of Candida albicans to denture base acrylic resin. The present study aimed to test the in vitro effect of traditional and novel fabrication methods on Candida albicans adhesion to denture base samples. Denture based acrylic discs were fabricated using: (i) computerized milling, (ii) 3D printing, (iii) heat curing, and (iv) cold curing. Discs were tested for surface roughness (Ra), hydrophobicity (contact angle), mucin adsorption (Bradford assay), and Candida albicans adhesion. 3D printing significantly increased microbial cell adhesion as compared with heat curing, and computerized milling significantly decreased it. These results were associated with mucin adsorption levels rather than surface roughness. Results suggest that 3D printing may increase the risk for developing denture stomatitis, whereas computerized milling may decrease it as compared with traditional heat curing denture base fabrication.
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