Two hemocyte lineages exist in silkworm larval hematopoietic organ.
Two hemocyte lineages exist in silkworm larval hematopoietic organ.
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DOI：
10.1371/journal.pone.0011816
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发表时间：
2010-07-28
期刊：
影响因子：
3.7
通讯作者：
Kamimura M
中科院分区：
文献类型：
--
作者：
Nakahara Y;Kanamori Y;Kiuchi M;Kamimura M
昆虫和脊椎动物一样，具有多种功能不同的血细胞形态，然而，除了黑腹果蝇之外，它们的造血谱系在很大程度上还未被探索。为了研究家蚕的造血谱系，我们在体内和体外研究了造血器官（HPO）中的血细胞前体细胞分化为四个成熟的血细胞亚群，即浆细胞、粒细胞、类卵细胞和球红细胞。将来自GFP表达转基因系的酶分散的HPO细胞植入正常幼虫的血腔中5天后，观察到分化为浆细胞、粒细胞和类卵细胞，但不分化为球红细胞。体外培养HPO细胞，浆细胞迅速出现，数天后出现具有酚氧化酶活性的类卵细胞。HPO细胞也能分化成少量粒细胞，但不能分化成球细胞。当功能成熟的浆细胞在体外培养，10天后观察到类卵母细胞。这些结果表明，HPO中的血细胞前体细胞首先分化为浆细胞，浆细胞进一步分化为类卵细胞。根据这些结果，我们提出B.桑血细胞可分为两个主要谱系：粒细胞谱系和浆细胞-类酒细胞谱系。本研究中无法确定球形红细胞的来源。我们建立了一个B幼虫期造血谱系的模型。桑。
Insects have multiple hemocyte morphotypes with different functions as do vertebrates, however, their hematopoietic lineages are largely unexplored with the exception of Drosophila melanogaster. To study the hematopoietic lineage of the silkworm, Bombyx mori, we investigated in vivo and in vitro differentiation of hemocyte precursors in the hematopoietic organ (HPO) into the four mature hemocyte subsets, namely, plasmatocytes, granulocytes, oenocytoids, and spherulocytes. Five days after implantation of enzymatically-dispersed HPO cells from a GFP-expressing transgenic line into the hemocoel of normal larvae, differentiation into plasmatocytes, granulocytes and oenocytoids, but not spherulocytes, was observed. When the HPO cells were cultured in vitro, plasmatocytes appeared rapidly, and oenocytoids possessing prophenol oxidase activity appeared several days later. HPO cells were also able to differentiate into a small number of granulocytes, but not into spherulocytes. When functionally mature plasmatocytes were cultured in vitro, oenocytoids were observed 10 days later. These results suggest that the hemocyte precursors in HPO first differentiate into plasmatocytes, which further change into oenocytoids. From these results, we propose that B. mori hemocytes can be divided into two major lineages, a granulocyte lineage and a plasmatocyte-oenocytoid lineage. The origins of the spherulocytes could not be determined in this study. We construct a model for the hematopoietic lineages at the larval stage of B. mori.
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影响因子：
0.9
作者：
Han, SS;Lee, MH;Yoe, SM
通讯作者：
Yoe, SM
影响因子：
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作者：
Crozatier M;Ubeda JM;Vincent A;Meister M
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DOI：
10.1006/bbrc.2001.5365
发表时间：
2001-08-10
影响因子：
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作者：
Kamimura, M;Nakahara, Y;Kiuchi, M
通讯作者：
Kiuchi, M
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作者：
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通讯作者：
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