Metagenomic analysis of the gut microbiota in piglets either challenged or not with enterotoxigenic Escherichia coli reveals beneficial effects of probiotics on microbiome composition, resistome, digestive function and oxidative stress responses.
Metagenomic analysis of the gut microbiota in piglets either challenged or not with enterotoxigenic Escherichia coli reveals beneficial effects of probiotics on microbiome composition, resistome, digestive function and oxidative stress responses.
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DOI：
10.1371/journal.pone.0269959
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
本研究使用宏基因组分析来研究仔猪的肠道微生物群和抗性组，所述仔猪被或未被肠致病性大肠杆菌（ETEC）攻击，并且接受或未接受微胶囊化益生菌的饮食补充。72头仔猪分为6组，分别接受非ETEC攻毒（第1-3组）或ETEC攻毒（第4-6组，在3周龄断奶后1周接受5 ml 109 CFU/ml致病性ETEC菌株L3.2）。在仔猪2、5、8、11和14日龄的五个时间点，第2组和第5组补充109 CFU/ml的多菌株益生菌（植物乳杆菌菌株22 F和25 F，以及乳酸片球菌72 N），而第4组接受109 CFU/ml的乳酸片球菌72 N。第3组在断奶仔猪饲料中接受300 mg/kg金霉素，以模拟商业条件。在2日龄时以及断奶后ETEC攻毒后12小时和14天采集直肠粪便样本进行宏基因组和耐药基因组分析。仔猪在42日龄时全部被安乐死。第2组和第5组中的仔猪富含几种理想的微生物家族，包括乳杆菌科、毛螺菌科和瘤胃球菌科，而第3组中的仔猪具有类杆菌科成员的增加，并且表现出tetW和tetQ基因的增加。第5组具有较少的铜和多杀生物剂抗性。移动的遗传元件IncQ 1和IncX 4是在断奶仔猪中最普遍的复制子。仅第6组和第3组分别检测到整合酶基因（intl）2类和3类。插入序列（IS）1380在第3组中普遍存在。与饮食摄入有关的IS 3和IS 30在第5组中的比例过高。此外，只有第5组显示出与解毒相关的基因，与其他组相比，与氧化应激、葡萄糖代谢和氨基酸代谢相关的基因富集。总的来说，宏基因组分析表明，采用多菌株益生菌可以改变肠道微生物群，减少耐药基因组，并促进与食物代谢相关的基因。
This study used metagenomic analysis to investigate the gut microbiota and resistome in piglets that were or were not challenged with enterotoxigenic Escherichia coli (ETEC) and had or had not received dietary supplementation with microencapsulated probiotics. The 72 piglets belonged to six groups that were either non-ETEC challenged (groups 1–3) or ETEC challenged (receiving 5ml of 109 CFU/ml pathogenic ETEC strain L3.2 one week following weaning at three weeks of age: groups 4–6). On five occasions at 2, 5, 8, 11, and 14 days of piglet age, groups 2 and 5 were supplemented with 109 CFU/ml of multi-strain probiotics (Lactiplantibacillus plantarum strains 22F and 25F, and Pediococcus acidilactici 72N) while group 4 received 109 CFU/ml of P. acidilactici 72N. Group 3 received 300mg/kg chlortetracycline in the weaner diet to mimic commercial conditions. Rectal faecal samples were obtained for metagenomic and resistome analysis at 2 days of age, and at 12 hours and 14 days after the timing of post-weaning challenge with ETEC. The piglets were all euthanized at 42 days of age. The piglets in groups 2 and 5 were enriched with several desirable microbial families, including Lactobacillaceae, Lachnospiraceae and Ruminococcaceae, while piglets in group 3 had increases in members of the Bacteroidaceae family and exhibited an increase in tetW and tetQ genes. Group 5 had less copper and multi-biocide resistance. Mobile genetic elements IncQ1 and IncX4 were the most prevalent replicons in antibiotic-fed piglets. Only groups 6 and 3 had the integrase gene (intl) class 2 and 3 detected, respectively. The insertion sequence (IS) 1380 was prevalent in group 3. IS3 and IS30, which are connected to dietary intake, were overrepresented in group 5. Furthermore, only group 5 showed genes associated with detoxification, with enrichment of genes associated with oxidative stress, glucose metabolism, and amino acid metabolism compared to the other groups. Overall, metagenomic analysis showed that employing a multi-strain probiotic could transform the gut microbiota, reduce the resistome, and boost genes associated with food metabolism.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
DOI：
10.1186/s12941-015-0100-6
发表时间：
2015-10-20
影响因子：
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作者：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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