A neuroendocrine mechanism of co-morbidity of depression-like behavior and myocardial injury in rats.
A neuroendocrine mechanism of co-morbidity of depression-like behavior and myocardial injury in rats.
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大鼠抑郁行为和心肌损伤合并症的神经内分泌机制。
DOI：
10.1371/journal.pone.0088427
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lingjia Q
中科院分区：
文献类型：
--
作者：
Xinxing W;Wei L;Lei W;Rui Z;Baoying J;Lingjia Q
抑郁症通常是一种复发性精神疾病。有证据表明抑郁症和心血管疾病是常见的共病，但具体的病理机制尚不清楚。本研究旨在探讨慢性不可预见性轻度应激（CIMs）所致抑郁对心肌损伤的影响，进一步阐明抑郁的生物学机制。大鼠被用作模型。手术共持续8周。经过4周的BASIS，治疗组大鼠表现出减少蔗糖的偏好和变化的分数在一个开放的领域测试，体重和5-HT在大脑中的含量相比，这些变量的值在控制。这些变化表明，抑郁样改变在BRAS大鼠，并证明抑郁模型的可行性。此外，心肌的病理变化和心肌细胞凋亡增加表明，心肌损伤发生在6周后，并在8周结束时显著增加。采用高效液相色谱-电子捕获检测法（HPLC-ECD）测定对照组和抑郁症模型组大鼠血浆5-羟色胺（5-HT）、去甲肾上腺素（NE）和肾上腺素（E）的含量，采用碘125-皮质醇放射免疫法测定血浆皮质酮（GC）的含量。结果表明，抑郁症大鼠5-HT含量降低，NE、E和GC含量升高，这些因素可能与抑郁症心肌损伤有关。采用正交实验设计，观察5-HT、NE和GC对体外培养心肌细胞存活率的影响。结果表明，5-HT是影响细胞存活的一个比GC和NE更重要的因素。结果表明，正常血中5-HT水平具有细胞保护作用。以5-HT减少、GC和NE增加为特征的神经内分泌紊乱介导了抑郁性心肌损伤的发生。
Depression is generally a recurrent psychiatric disorder. Evidence shows that depression and cardiovascular diseases are common comorbid conditions, but the specific pathological mechanisms remain unclear. The purpose of this study is to determine the effects of depression induced by chronic unpredictable mild stress (CUMS) on myocardial injury and to further elucidate the biological mechanism of depression. Rats were used as a model. The CUMS procedure lasted for a total of 8 weeks. After 4 weeks of CUMS, treated rats exhibited a reduced sucrose preference and changes in scores on an open field test, body weight and content of 5-HT in the brain as compared with the values of these variables in controls. These changes indicated depression-like changes in CUMS rats and demonstrated the feasibility of the depression model. In addition, pathological changes in the myocardium and increased cardiomyocyte apoptosis demonstrated that myocardial injury had occurred after 6 weeks of CUMS and had increased significantly by the end of 8 weeks of CUMS. Plasma serotonin (5-HT), norepinephrine (NE) and epinephrine (E), all depression-related neuroendocrine factors, were measured by HPLC-ECD techniques, and the content of plasma corticosterone (GC) was evaluated by an I125–cortisol radioactivity immunoassay in control and CUMS rats. The results indicated that 5-HT had decreased, whereas NE, E and GC had increased in CUMS rats, and these factors might be associated with depression-induced myocardial injury. The effects of 5-HT, NE and GC on the survival rate of cultured cardiomyocytes were determined using an orthogonal design. The results showed that 5-HT was a more important factor affecting cell survival than GC or NE. The results suggested that normal blood levels of 5-HT had a cytoprotective effect. The neuroendocrine disorders characterized by decreased 5-HT combined with increased GC and NE mediated the occurrence of depression-induced myocardial injury.
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影响因子：
8.2
作者：
Choleris, E;Thomas, AW;Prato, FS
通讯作者：
Prato, FS
影响因子：
8.8
作者：
Bravo, Lidia;Antonio Mico, Juan;Berrocoso, Esther
通讯作者：
Berrocoso, Esther
影响因子：
5.6
作者：
Goldstein, Inbal;Lax, Elad;Lichtstein, David
通讯作者：
Lichtstein, David
影响因子：
10.6
作者：
Keedwell, Paul A.;Chapman, Rosanna;Jones, Derek K.
通讯作者：
Jones, Derek K.
DOI：
10.2174/1874192401307010099
发表时间：
2013
期刊：
The open cardiovascular medicine journal
影响因子：
--
作者：
Delui MH;Yari M;Khouyinezhad G;Amini M;Bayazi MH
通讯作者：
Bayazi MH