Comparative proteomics reveals mechanisms that underlie insecticide resistance in Culex pipiens pallens Coquillett.
Comparative proteomics reveals mechanisms that underlie insecticide resistance in Culex pipiens pallens Coquillett.
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比较蛋白质组学揭示淡色库蚊对杀虫剂产生抗性的机制
DOI：
10.1371/journal.pntd.0009237
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发表时间：
2021-03
影响因子：
3.8
通讯作者：
Gong M
中科院分区：
文献类型：
--
作者：
Zhang C;Shi Q;Li T;Cheng P;Guo X;Song X;Gong M
以化学杀虫剂为基础的蚊子控制被认为是当前控制蚊媒疾病的全球战略的重要组成部分。不幸的是，重要媒介蚊种的抗药性的发展危及以杀虫剂为基础的蚊子控制的有效性。与靶点抗性不同的是，其他机制还远未完全了解。获得了淡色库蚊对氯氰菊酯抗性、残杀威抗性、二甲基二氯乙烯基磷酸盐抗性和敏感品系的整体蛋白质谱，并利用相对和绝对定量标记结合高效液相色谱/串联质谱仪分析了蛋白质组的差异。经过25代杀虫剂筛选后，淡色库蚊敏感品系的抗性水平有所提高，通过iTRAQ数据分析，检测到2,502个蛋白质，其中1,513个蛋白质在杀虫剂选择品系中与敏感品系相比差异表达。最后，对中肠差异蛋白表达谱进行了分析，选择了62个蛋白质分别采用iTRAQ和平行反应监测策略进行差异表达验证。对3种杀虫剂菌株的适应选择的iTRAQ图谱结合中肠图谱显示，淡色库蚊对多种杀虫剂的抗性机制同时起作用。淡色库蚊的重要分子资源已被开发，潜在的候选杀虫剂涉及代谢抗性和降低穿透或隔离杀虫剂。针对已鉴定的代谢靶点，如角质层蛋白、细胞色素P450、谷胱甘肽S转移酶、核糖体蛋白等的RNA干扰研究，将为更好地了解淡色库蚊抗药性的遗传基础奠定基础。对CX敏感株的全球蛋白质谱进行了比较。淡色库蚊和抗氯氰菊酯、残杀威和抗二甲基二氯乙烯基磷酸盐的品系经过25代不同化学杀虫剂家族的筛选，发现多种机制同时作用于Cx抗性蚊虫。淡色库蚊，包括降低杀虫剂的渗透率或隔离杀虫剂的机制，或通过角质层蛋白质水平或解毒酶(P450和谷胱甘肽S转移酶)活性的细微变化增加杀虫剂的生物降解性。
Mosquito control based on chemical insecticides is considered as an important element of the current global strategies for the control of mosquito-borne diseases. Unfortunately, the development of insecticide resistance of important vector mosquito species jeopardizes the effectiveness of insecticide-based mosquito control. In contrast to target site resistance, other mechanisms are far from being fully understood. Global protein profiles among cypermethrin-resistant, propoxur-resistant, dimethyl-dichloro-vinyl-phosphate-resistant and susceptible strain of Culex pipiens pallens were obtained and proteomic differences were evaluated by using isobaric tags for relative and absolute quantification labeling coupled with liquid chromatography/tandem mass spectrometric analysis. A susceptible strain of Culex pipiens pallens showed elevated resistance levels after 25 generations of insecticide selection, through iTRAQ data analysis detected 2,502 proteins, of which 1,513 were differentially expressed in insecticide-selected strains compared to the susceptible strain. Finally, midgut differential protein expression profiles were analyzed, and 62 proteins were selected for verification of differential expression using iTRAQ and parallel reaction monitoring strategy, respectively. iTRAQ profiles of adaptation selection to three insecticide strains combined with midgut profiles revealed that multiple insecticide resistance mechanisms operate simultaneously in resistant insects of Culex pipiens pallens. Significant molecular resources were developed for Culex pipiens pallens, potential candidates were involved in metabolic resistance and reducing penetration or sequestering insecticide. Future research that is targeted towards RNA interference of the identified metabolic targets, such as cuticular proteins, cytochrome P450s, glutathione S-transferases and ribosomal proteins proteins and biological pathways (drug metabolism—cytochrome P450, metabolism of xenobiotics by cytochrome P450, oxidative phosphorylation, ribosome) could lay the foundation for a better understanding of the genetic basis of insecticide resistance in Culex pipiens pallens. Global protein profiles were compared among a susceptible strain of Cx. pipiens pallens and strains that were cypermethrin-resistant, propoxur-resistant, and dimethyl-dichloro-vinyl-phosphate-resistant after 25 generations of selection by distinct chemical insecticide families, multiple mechanisms were found to operate simultaneously in resistant mosquitoes of Cx. pipiens pallens, including mechanisms to lower penetration of or sequester the insecticide or to increase biodegradation of the insecticide via subtle alterations in either the cuticular protein levels or the activities of detoxification enzymes (P450s and glutathione S-transferases).
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影响因子：
3.7
作者：
Chen L;Zhong D;Zhang D;Shi L;Zhou G;Gong M;Zhou H;Sun Y;Ma L;He J;Hong S;Zhou D;Xiong C;Chen C;Zou P;Zhu C;Yan G
通讯作者：
Yan G
影响因子：
2.1
作者：
Baker, Nicholas E.;Kale, Abhijit
通讯作者：
Kale, Abhijit
影响因子：
3
作者：
Antonio-Nkondjio C;Poupardin R;Tene BF;Kopya E;Costantini C;Awono-Ambene P;Wondji CS
通讯作者：
Wondji CS
影响因子：
3.2
作者：
Dang K;Doggett SL;Veera Singham G;Lee CY
通讯作者：
Lee CY
影响因子：
2.2
作者：
APPERSON, CS;GEORGHIOU, GP
通讯作者：
GEORGHIOU, GP