Both silencing- and over-expression of pepper CaATG8c gene compromise plant tolerance to heat and salt stress
Both silencing- and over-expression of pepper CaATG8c gene compromise plant tolerance to heat and salt stress
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辣椒CaATG8c基因的沉默和过度表达都会损害植物对热和盐胁迫的耐受性
DOI：
10.1016/j.envexpbot.2017.06.009
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发表时间：
2017-09
影响因子：
5.7
通讯作者：
Lu Minghui
中科院分区：
文献类型：
--
作者：
Zhai Yufei;Wang Hu;Liang Minmin;Lu Minghui
自噬是一种保护细胞免受不良环境影响的保守生存机制，而自噬相关蛋白8 （Autophagy -related protein 8, ATG8）作为细胞自噬过程的重要组成部分，在自噬过程中起着关键作用。然而，ATG8对植物环境胁迫耐受性的贡献尚不明确。为了深入了解ATG8基因是否以及如何在植物适应不利环境条件中起作用，我们对辣椒（Capsicum annuumL.）中的ATG8基因ecaatg8c进行了特征分析，发现推断出的caatg8c基因氨基酸序列包含ATG8功能的保守结构域和残基。正常情况下，caatg8c在辣椒的所有组织器官中均有表达，其亚细胞表达位于洋葱表皮细胞的细胞质中。caatg8基因的沉默表达加剧了辣椒幼苗叶绿素降解、MDA积累和SOD活性降低，从而使辣椒幼苗对热盐胁迫更加敏感。出乎意料的是，caatg8cgene的过表达还通过抑制主根的生长和次生根的产生，干扰胁迫应答基因的表达，降低了拟南芥对热盐胁迫的耐受性。我们的研究结果表明，caatg8cgene的沉默和过表达使植物对热盐胁迫更敏感。此外，caatg8沉默的可能机制被推测为去除积累ROS的能力降低，而基因过表达则可能是ROS信号受到干扰。我们认为caatg8c的稳定和及时表达可能对植物适应不利环境至关重要。
Autophagy is well-known as a conserved survival mechanism that protects cells from adverse environments, and autophagy-related protein 8 (ATG8) plays a key role in autophagy process as an essential component. However, the contribution of ATG8 to plant tolerance to environmental stresses is ambiguous. To gain insight into whether and howATG8gene functions in plant adaptation to adverse environment conditions, we characterized anATG8geneCaATG8cfrom pepper (Capsicum annuumL.), and found that the deduced amino acid sequence ofCaATG8cgene contained the conserved necessary domains and residue for ATG8 functions. Under normal condition, the expression ofCaATG8cwas detected in all pepper tissues/organs, and its subcellular expression was located to the cytoplasm in onion epidermal cells. The silencing-expression ofCaATG8crendered pepper seedlings more sensitive to heat and salt stress by aggravating the chlorophyll degradation, MDA accumulation and SOD activity decrease. Unexpectedly, the over-expression ofCaATG8cgene also reducedArabidopsistolerance to heat and salt stress by inhibiting the growth of primary roots and the generation of secondary roots, and disturbing the expression of stress response genes. Our results suggested that both silencing- and over-expression ofCaATG8cgene conferred plants more sensitivity to heat and salt stress. Furthermore, the possible mechanism forCaATG8csilencing is speculated to the reduced ability to remove accumulated ROS, whereas for gene over-expression, it is supposed to the disturbance of ROS signal. We propose that the stable and timely expression ofCaATG8cmay be important for plant adaption to adverse environments.
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影响因子：
13.3
作者：
Chen L;Liao B;Qi H;Xie LJ;Huang L;Tan WJ;Zhai N;Yuan LB;Zhou Y;Yu LJ;Chen QF;Shu W;Xiao S
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DOI：
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影响因子：
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作者：
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7.2
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影响因子：
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