Identification of transcriptome-derived microsatellite markers and their association with the growth performance of the mud crab (Scylla paramamosain).
Identification of transcriptome-derived microsatellite markers and their association with the growth performance of the mud crab (Scylla paramamosain).
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DOI：
10.1371/journal.pone.0089134
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ma L
中科院分区：
文献类型：
--
作者：
Ma H;Jiang W;Liu P;Feng N;Ma Q;Ma C;Li S;Liu Y;Qiao Z;Ma L
初步分离了转录组序列文库中的微卫星标记，并对其在青蟹野生种群中的遗传变异进行了研究。然后，我们测试了这些微卫星标记与副毛链霉菌生长性能之间的关系。共检测到129个多态微卫星标记，每个座位的观测杂合度在0.19~1.00之间，期望杂合度在0.23~0.96之间，多态信息含量(PIC)在0.21~0.95之间。在这些微卫星标记中，有30个在第一代家系的96个全同胞个体中表现出多态性。统计分析表明，3个微卫星标记与副甲藻的12个生长性状显著相关。在这3个标记中，Scpa36基因座与甲长、腹宽(AW)、体高(BH)、爪固定指长、爪固定指宽、爪固定指高、2周足节长、3周足节长(MLP3)等8个生长性状显著相关(P<；0.05)。Scpa75基因座与5个生长性状显著相关，即内甲宽、AW、8号甲宽、2号侧棘距(DLS2)和MLP3(P<；0.05)。Spm30基因座与BH、DLS2和体重显著相关(P<；0.05)。进一步分析表明，Scpa36上的BC、Scpa75上的BC和BD、Spm30上的AC等一系列基因型别在副毛链霉菌的生长性状选择中具有很大的潜力。这些发现将有助于群体保护遗传学和分子标记辅助选择育种的发展。
Microsatellite markers from a transcriptome sequence library were initially isolated, and their genetic variation was characterized in a wild population of the mud crab (Scylla paramamosain). We then tested the association between these microsatellite markers and the growth performance of S. paramamosain. A total of 129 polymorphic microsatellite markers were identified, with an observed heterozygosity ranging from 0.19 to 1.00 per locus, an expected heterozygosity ranging from 0.23 to 0.96 per locus, and a polymorphism information content (PIC) ranging from 0.21 to 0.95 per locus. Of these microsatellite markers, 30 showed polymorphism in 96 full-sib individuals of a first generation family. Statistical analysis indicated that three microsatellite markers were significantly associated with 12 growth traits of S. paramamosain. Of these three markers, locus Scpa36 was significantly associated with eight growth traits, namely, carapace length, abdomen width (AW), body height (BH), fixed finger length of the claw, fixed finger width of the claw, fixed finger height of the claw, meropodite length of pereiopod 2, and meropodite length of pereiopod 3 (MLP3) (P<0.05). Locus Scpa75 was significantly associated with five growth traits, namely, internal carapace width, AW, carapace width at spine 8, distance between lateral spine 2 (DLS2), and MLP3 (P<0.05). Locus Spm30 was significantly associated with BH, DLS2, and body weight (P<0.05). Further analysis suggested a set of genotypes (BC at Scpa36, BC and BD at Scpa75, and AC at Spm30) that have great potential in the selection of S. paramamosain for growth traits. These findings will facilitate the development of population conservation genetics and molecular marker-assisted selective breeding of S. paramamosain and other closely related species.
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影响因子：
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影响因子：
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