A murine model of myocardial ischemia-reperfusion injury through ligation of the left anterior descending artery.
A murine model of myocardial ischemia-reperfusion injury through ligation of the left anterior descending artery.
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DOI：
10.3791/51329
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发表时间：
2014-04-10
期刊：
影响因子：
--
通讯作者：
Weisleder N
中科院分区：
文献类型：
--
作者：
Xu Z;Alloush J;Beck E;Weisleder N
急性或慢性心肌梗死（MI）是导致高发病率和死亡率的心血管事件。建立MI期间的病理机制和开发有效的治疗方法需要可重复地模拟临床发病率并反映与MI相关的病理生理变化的方法。在这里，我们描述了一种手术方法，以诱导MI小鼠模型，可用于短期缺血再灌注（I/R）损伤以及永久结扎。该方法的主要优点是便于左前降支动脉（LAD）的定位，以允许该动脉的准确结扎，从而在小鼠心脏的左心室中诱导缺血。结扎线在LAD上的准确定位增加了梗死面积的可重复性，从而产生更可靠的结果。结扎线放置的更高精度将改善模拟小鼠MI的标准手术方法，从而减少统计学相关研究所需的实验动物数量，并提高我们对MI后心功能障碍机制的理解。该MI小鼠模型也可用于MI后靶向心肌损伤的治疗的临床前测试。
Acute or chronic myocardial infarction (MI) are cardiovascular events resulting in high morbidity and mortality. Establishing the pathological mechanisms at work during MI and developing effective therapeutic approaches requires methodology to reproducibly simulate the clinical incidence and reflect the pathophysiological changes associated with MI. Here, we describe a surgical method to induce MI in mouse models that can be used for short term ischemia-reperfusion (I/R) injury as well as permanent ligation. The major advantage of this method is to facilitate location of the left anterior descending artery (LAD) to allow for accurate ligation of this artery to induce ischemia in the left ventricle of the mouse heart. Accurate positioning of the ligature on the LAD increases reproducibility of infarct size and thus produces more reliable results. Greater precision in placement of the ligature will improve the standard surgical approaches to simulate MI in mice, thus reducing the number of experimental animals necessary for statistically relevant studies and improving our understanding of the mechanisms producing cardiac dysfunction following MI. This mouse model of MI is also useful for the preclinical testing of treatments targeting myocardial damage following MI.
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