Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic β-cells.
Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic β-cells.
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DOI：
10.3164/jcbn.10-119
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发表时间：
2011-05
影响因子：
2.4
通讯作者：
Yagasaki K
中科院分区：
文献类型：
--
作者：
Minakawa M;Kawano A;Miura Y;Yagasaki K
白藜芦醇是一种存在于葡萄皮和红葡萄酒中的植物抗毒素，已被证明具有广泛的健康促进活性，包括抗糖尿病特性。在本研究中，我们研究了白藜芦醇在2型糖尿病小鼠和细胞培养系统中的作用。在体外培养的L 6肌管中，我们从胰岛素信号和AMP激活的蛋白激酶信号两个方面研究了白藜芦醇对葡萄糖摄取和葡萄糖转运蛋白4向质膜转运的影响。在培养的RIN-5 F细胞中，我们检查了白藜芦醇是否会保护胰腺来源的β细胞免受氧化应激。白藜芦醇显著抑制db/db小鼠空腹血糖水平和血清甘油三酯和脂质过氧化物水平的升高。白藜芦醇通过激活胰岛素信号和AMP激活的蛋白激酶信号刺激葡萄糖摄取和葡萄糖转运蛋白4转位。白藜芦醇对糖基化终产物诱导的胰腺β细胞氧化应激和凋亡具有保护作用。根据这些结果，提示白藜芦醇通过刺激肌肉中胰岛素依赖性和非胰岛素依赖性葡萄糖摄取以及通过保护胰腺β细胞免受晚期糖基化终产物诱导的氧化应激和凋亡而显示出抗糖尿病作用。
Resveratrol, a phytoalexin present in the skin of grapes and red wine, has been demonstrated to possess a wide range of health promoting activities including anti-diabetic properties. In the present study, we investigated the effect of resveratrol in both type 2 diabetic mice and cell culture systems. In cultured L6 myotubes, we studied the effect of resveratrol on glucose uptake and translocation of glucose transporter 4 to plasma membrane from the aspects of insulin signaling and AMP-activated protein kinase signaling. In cultured RIN-5F cells, we examined whether resveratrol would protect the pancreas-derived β-cells from oxidative stress. Resveratrol significantly suppressed the elevation in the fasting blood glucose level and the serum triglyceride and lipid peroxide levels in db/db mice. Resveratrol stimulated glucose uptake and glucose transporter 4 translocation by activating both insulin signaling and AMP-activated protein kinase signaling. Moreover, resveratrol could protect pancreatic β-cells from advanced glycation end products-induced oxidative stress and apoptosis. From these results, resveratrol is suggested to show anti-diabetic effect by stimulating both insulin-dependent and -independent glucose uptake in muscles and by protecting pancreatic β-cells from advanced glycation end products-induced oxidative stress and apoptosis.
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影响因子：
7.4
作者：
Lenzen, S;Drinkgern, J;Tiedge, M
通讯作者：
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影响因子：
4.3
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