Mesoscopic patterns of neural activity support songbird cortical sequences.
Mesoscopic patterns of neural activity support songbird cortical sequences.
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DOI：
10.1371/journal.pbio.1002158
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发表时间：
2015-06
期刊：
影响因子：
9.8
通讯作者：
Gardner TJ
中科院分区：
文献类型：
--
作者：
Markowitz JE;Liberti WA 3rd;Guitchounts G;Velho T;Lois C;Gardner TJ
在不同物种和行为背景下，整个脊椎动物前脑都可以找到时间锁定的神经活动序列。从啮齿动物海马体中的“时间细胞”到控制运动的皮层活动，时间序列的生成是许多形式的学习行为不可或缺的一部分。然而，序列生成的机制尚不清楚。在这里，我们描述了支持稀疏神经活动序列的鸣禽前运动皮层微电路的空间和时间组织。多通道电生理学和钙成像揭示前运动皮层的神经活动与 100 µm 的长度尺度相关。在这个长度范围内，基底神经节投射的兴奋性神经元平均在局部 30 Hz 网络节律的特定阶段放电。这些结果表明，运动前皮层活动在时间和空间上是不均匀的，并且介观动力学模式是控制歌曲的神经序列生成的基础。鸣禽歌唱行为期间的皮层运动前活动在 100 µm 的长度范围内时空相关，神经元和中间神经元以 30 Hz 网络节律的相反相位放电。阅读概要。在不同物种和行为环境中，整个脊椎动物前脑中都存在“时间细胞”。在刻板行为期间，这些神经元在精确的时间点稀疏地放电；然而，神经回路如何支持这种非凡的特性尚不清楚。在这里，我们描述了鸟类歌唱所需的前运动神经元回路（“HVC”）以支持稀疏神经活动序列的空间和时间方式组织。在唱歌期间，一类主要神经元会在 30 Hz 网络节奏的特定阶段放电。使用头戴式显微镜的荧光成像显示，附近主要神经元的钙活动与 100 μm 长度尺度的时间相关。因此，HVC 网络节律的相位或精确计时的变化可以提供协调主要神经元活动的机制。这一观察结果表明，主细胞的计时受到神经活动的介观时空模式的支持。
Time-locked sequences of neural activity can be found throughout the vertebrate forebrain in various species and behavioral contexts. From “time cells” in the hippocampus of rodents to cortical activity controlling movement, temporal sequence generation is integral to many forms of learned behavior. However, the mechanisms underlying sequence generation are not well known. Here, we describe a spatial and temporal organization of the songbird premotor cortical microcircuit that supports sparse sequences of neural activity. Multi-channel electrophysiology and calcium imaging reveal that neural activity in premotor cortex is correlated with a length scale of 100 µm. Within this length scale, basal-ganglia–projecting excitatory neurons, on average, fire at a specific phase of a local 30 Hz network rhythm. These results show that premotor cortical activity is inhomogeneous in time and space, and that a mesoscopic dynamical pattern underlies the generation of the neural sequences controlling song. Cortical premotor activity during singing behavior in songbirds is correlated spatiotemporally over a length scale of 100 µm, with neurons and interneurons firing at opposite phases of a 30 Hz network rhythm. Read the Synopsis. “Time cells” can be found throughout the vertebrate forebrain in various species and behavioral contexts. These neurons fire sparsely at precise times during a stereotyped behavior; however, how a neural circuit supports this remarkable property is not known. Here, we describe that the premotor neuronal circuit that is required in birds for singing—“HVC”—is organized in a spatial and temporal manner that supports sparse sequences of neural activity. During song, one class of principal neuron fires during a specific phase of a 30 Hz network rhythm. Fluorescent imaging using head-mounted microscopes reveals that calcium activity in nearby principal neurons is correlated in time with a length scale of 100 μm. Thus, variations in the phase or precise timing of the network rhythm across HVC can provide a mechanism for coordinating the activity of principal neurons. This observation suggests that the timing of principal cells is supported by a mesoscopic spatiotemporal pattern of neural activity.
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影响因子：
25
作者：
通讯作者：
--
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DOI：
10.1038/nrn3241
发表时间：
2012-05-18
期刊：
Nature reviews. Neuroscience
影响因子：
--
作者：
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通讯作者：
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影响因子：
3
作者：
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通讯作者：
Nick, Teresa A.
影响因子：
4
作者：
Guitchounts G;Markowitz JE;Liberti WA;Gardner TJ
通讯作者：
Gardner TJ
影响因子：
5.3
作者：
Glaze, CM;Troyer, TW
通讯作者：
Troyer, TW