Diversity of Interneurons in the Dorsal Striatum Revealed by Single-Cell RNA Sequencing and PatchSeq.
Diversity of Interneurons in the Dorsal Striatum Revealed by Single-Cell RNA Sequencing and PatchSeq.
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DOI：
10.1016/j.celrep.2018.07.053
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发表时间：
2018-08-21
期刊：
影响因子：
8.8
通讯作者：
Hjerling-Leffler J
中科院分区：
文献类型：
--
作者：
Muñoz-Manchado AB;Bengtsson Gonzales C;Zeisel A;Munguba H;Bekkouche B;Skene NG;Lönnerberg P;Ryge J;Harris KD;Linnarsson S;Hjerling-Leffler J
Striatal locally projecting neurons, or interneurons, act on nearby circuits and shape functional output to the rest of the basal ganglia. We performed single-cell RNA sequencing of striatal cells enriching for interneurons. We find seven discrete interneuron types, six of which are GABAergic. In addition to providing specific markers for the populations previously described, including those expressing Sst/Npy, Th, Npy without Sst, and Chat, we identify two small populations of cells expressing Cck with or without Vip. Surprisingly, the Pvalb-expressing cells do not constitute a discrete cluster but rather are part of a larger group of cells expressing Pthlh with a spatial gradient of Pvalb expression. Using PatchSeq, we show that Pthlh cells exhibit a continuum of electrophysiological properties correlated with expression of Pvalb. Furthermore, we find significant molecular differences that correlate with differences in electrophysiological properties between Pvalb-expressing cells of the striatum and those of the cortex. Single-cell RNA sequencing of striatal interneurons reveals seven main classes Subclass-specific latent factor analysis reveals gradients of gene expression Pvalb neurons are not a discrete class but belong to a class expressing Pthlh Interneuron classes vary in their similarity to counterparts from cortex/hippocampus Muñoz-Manchado et al. describe the molecular and electrophysiological diversity of interneurons from the striatum. They find that in contrast to many other brain regions, cells expressing the gene Pvalb are not a discrete class of cells. This affects the interpretation of studies on striatal networks and their function.
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影响因子：
46.9
作者：
Fuzik J;Zeisel A;Máté Z;Calvigioni D;Yanagawa Y;Szabó G;Linnarsson S;Harkany T
通讯作者：
Harkany T
影响因子：
25
作者：
De Marco García NV;Priya R;Tuncdemir SN;Fishell G;Karayannis T
通讯作者：
Karayannis T
影响因子：
9.8
作者：
Harris KD;Hochgerner H;Skene NG;Magno L;Katona L;Bengtsson Gonzales C;Somogyi P;Kessaris N;Linnarsson S;Hjerling-Leffler J
通讯作者：
Hjerling-Leffler J
DOI：
10.1126/science.aab3415
发表时间：
2015-09-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dehorter N;Ciceri G;Bartolini G;Lim L;del Pino I;Marín O
通讯作者：
Marín O
影响因子：
13.9
作者：
Fishell G;Rudy B
通讯作者：
Rudy B