Analysis of proteome profile in germinating soybean seed, and its comparison with rice showing the styles of reserves mobilization in different crops.
Analysis of proteome profile in germinating soybean seed, and its comparison with rice showing the styles of reserves mobilization in different crops.
复制标题
DOI：
10.1371/journal.pone.0056947
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Yang P
中科院分区：
文献类型：
--
作者：
Han C;Yin X;He D;Yang P
种子萌发是一个复杂的生理过程，在这一过程中，营养物质储备发生了动员。在不同的作物中，这一事件可能由不同的调控和代谢途径介导。蛋白质组分析已被证明是一种有效的方法，可以帮助我们构建这些途径。然而，迄今为止还没有在大豆发芽种子中进行这样的研究。采用一维凝胶电泳-液相色谱-串联质谱技术对大豆萌发种子进行蛋白质组分析。并对水稻和大豆发芽种子进行了综合比较。共鉴定出764个蛋白质，分属于14个功能组，其中代谢相关蛋白质是最大的功能组。对蛋白质和途径的深入分析表明，大豆种子萌发过程中，脂类通过脂氧合酶依赖途径降解，蛋白质通过蛋白酶和26 S蛋白酶体系统降解，脂氧合酶在快速动员大豆种子贮藏物质过程中还能帮助清除活性氧。水稻和大豆萌发种子蛋白质组的差异表明，每种作物在萌发过程中有不同的储备动员机制。不同作物种子在萌发过程中，不同的贮藏量在未被完全利用之前就转化为淀粉。本研究是迄今为止第一次对萌发大豆种子蛋白质组进行全面分析。本文所提供的数据将有助于我们更好地了解吸胀大豆种子萌发前的生理生化状况。与萌发水稻种子的蛋白质谱的比较为我们提供了新的见解，在作物种子萌发过程中的营养储备动员。
Seed germination is a complex physiological process during which mobilization of nutrient reserves happens. In different crops, this event might be mediated by different regulatory and metabolic pathways. Proteome profiling has been proved to be an efficient way that can help us to construct these pathways. However, no such studies have been performed in soybean germinating seeds up to date. Proteome profiling was conducted through one-dimensional gel electrophoresis followed by liquid chromatography and tandem mass spectrometry strategy in the germinating seeds of soybean (glycine max). Comprehensive comparisons were also carried out between rice and soybean germinating seeds. 764 proteins belonging to 14 functional groups were identified and metabolism related proteins were the largest group. Deep analyses of the proteins and pathways showed that lipids were degraded through lipoxygenase dependent pathway and proteins were degraded through both protease and 26S proteosome system, and the lipoxygenase could also help to remove the reactive oxygen species during the rapid mobilization of reserves of soybean germinating seeds. The differences between rice and soybean germinating seeds proteome profiles indicate that each crop species has distinct mechanism for reserves mobilization during germination. Different reserves could be converted into starches before they are totally utilized during the germination in different crops seeds. This study is the first comprehensive analysis of proteome profile in germinating soybean seeds to date. The data presented in this paper will improve our understanding of the physiological and biochemical status in the imbibed soybean seeds just prior to germination. Comparison of the protein profile with that of germinating rice seeds gives us new insights on mobilization of nutrient reserves during the germination of crops seeds.
登录
查看更多内容
影响因子：
4.3
作者：
Lu, Tian-Cong;Meng, Ling-Bo;Wang, Bai-Chen
通讯作者：
Wang, Bai-Chen
DOI：
10.1016/j.bbapap.2011.05.004
发表时间：
2011-09-01
影响因子：
3.2
作者：
Kim, Hyun Tae;Choi, Ung-Kyu;Kwon, Oh-Shin
通讯作者：
Kwon, Oh-Shin
影响因子：
3.4
作者：
He, Dongli;Han, Chao;Yang, Pingfang
通讯作者：
Yang, Pingfang
影响因子：
5.1
作者：
Howell, KA;Millar, AH;Whelan, J
通讯作者：
Whelan, J
影响因子：
7.4
作者：
Meyer, Louis J.;Gao, Jianjiong;Thelen, Jay J.
通讯作者：
Thelen, Jay J.