Response of Arabidopsis primary metabolism and circadian clock to low night temperature in a natural light environment.
Response of Arabidopsis primary metabolism and circadian clock to low night temperature in a natural light environment.
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DOI：
10.1093/jxb/ery276
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发表时间：
2018-09-14
影响因子：
6.9
通讯作者：
Stitt M
中科院分区：
文献类型：
--
作者：
Annunziata MG;Apelt F;Carillo P;Krause U;Feil R;Koehl K;Lunn JE;Stitt M
在自然光环境下生长的拟南芥植物的中枢代谢和生物钟受到光和温度共变的影响。植物在一天之内和日复一日地暴露在不同的辐照度和温度下。我们之前在春分的一个温控温室中研究了多云和晴天的代谢[日光照积分（DLI）为7 mol m−2 d−1和12 mol m−2 d−1]。除了氨基酸在自然光条件下较低外，Diel代谢物谱主要是在受控环境室中类似DLIs的正弦模拟中捕获的。我们现在扩展了DLI12的研究，通过研究在自然光照条件下的新陈代谢，包括在凉爽的夜晚。淀粉未完全转化，花青素和脯氨酸积累，蛋白质含量上升。氨基酸含量不降反升。中枢代谢的连通性在变光条件下下降，但温度变化不会进一步减弱。我们提出，当光和温度共变时，细胞代谢运行得更好。我们还比较了10个昼夜节律钟基因在这种温度可变制度下与温度控制的自然和正弦光制度下的转录丰度。尽管有温度补偿，但在变温度处理下，黎明期和白昼期基因以及GIGANTEA的峰值时间和丰度略有改变。这可能会延迟黎明生物钟的活动，直到温度上升到足以支持快速代谢和光合作用。
Central metabolism and the circadian clock are impacted by co-variation in light and temperature in Arabidopsis plants grown in a natural light environment. Plants are exposed to varying irradiance and temperature within a day and from day to day. We previously investigated metabolism in a temperature-controlled greenhouse at the spring equinox on both a cloudy and a sunny day [daily light integral (DLI) of 7 mol m−2 d−1 and 12 mol m−2 d−1]. Diel metabolite profiles were largely captured in sinusoidal simulations at similar DLIs in controlled-environment chambers, except that amino acids were lower in natural light regimes. We now extend the DLI12 study by investigating metabolism in a natural light regime with variable temperature including cool nights. Starch was not completely turned over, anthocyanins and proline accumulated, and protein content rose. Instead of decreasing, amino acid content rose. Connectivity in central metabolism, which decreased in variable light, was not further weakened by variable temperature. We propose that diel metabolism operates better when light and temperature are co-varying. We also compared transcript abundance of 10 circadian clock genes in this temperature-variable regime with the temperature-controlled natural and sinusoidal light regimes. Despite temperature compensation, peak timing and abundance for dawn- and day-phased genes and GIGANTEA were slightly modified in the variable temperature treatment. This may delay dawn clock activity until the temperature rises enough to support rapid metabolism and photosynthesis.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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