Functional Disruption of a Chloroplast Pseudouridine Synthase Desensitizes Arabidopsis Plants to Phosphate Starvation.
Functional Disruption of a Chloroplast Pseudouridine Synthase Desensitizes Arabidopsis Plants to Phosphate Starvation.
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叶绿体假尿苷合酶的功能破坏使拟南芥植物对磷酸盐饥饿不敏感
DOI：
10.3389/fpls.2017.01421
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发表时间：
2017
影响因子：
5.6
通讯作者：
Liu D
中科院分区：
文献类型：
--
作者：
Lu S;Li C;Zhang Y;Zheng Z;Liu D
磷酸盐缺乏是农业和自然生态系统中常见的植物营养胁迫。植物通过触发一系列生化、生理和发育变化来应对环境中的Pi饥饿，从而增加生存和生长。然而，决定植物对Pi饥饿敏感的关键因素尚不清楚。在这项研究中，我们鉴定了一个拟南芥突变体dps1，它对Pi饥饿的敏感性大大降低。dps1表型是由先前表征的SVR1（抑制变异1）基因突变引起的，该基因编码叶绿体定位的假尿嘧啶合成酶。SVR1突变导致叶绿体rRNA生物发生缺陷，从而减少叶绿体翻译。另一个突变体rps5，包含叶绿体核糖体蛋白rps5的突变，并减少了叶绿体翻译，也显示出对Pi饥饿的敏感性降低。此外，林可霉素（一种叶绿体翻译的化学抑制剂）处理的野生型植物表现出与dps1和rps5相似的生长表型和Pi饥饿反应。这些结果表明，受损的叶绿体翻译使植物对Pi饥饿脱敏。结合先前发表的研究结果表明，叶片光合作用的增强增强了植物对Pi饥饿的响应，我们认为dps1、rps5和林可霉素处理的植物对Pi饥饿的响应降低是由于它们对环境中Pi输入的需求减少。
Phosphate (Pi) deficiency is a common nutritional stress of plants in both agricultural and natural ecosystems. Plants respond to Pi starvation in the environment by triggering a suite of biochemical, physiological, and developmental changes that increase survival and growth. The key factors that determine plant sensitivity to Pi starvation, however, are unclear. In this research, we identified an Arabidopsis mutant, dps1, with greatly reduced sensitivity to Pi starvation. The dps1 phenotypes are caused by a mutation in the previously characterized SVR1 (SUPPRESSION OF VARIAGATION 1) gene, which encodes a chloroplast-localized pseudouridine synthase. The mutation of SVR1 results in defects in chloroplast rRNA biogenesis, which subsequently reduces chloroplast translation. Another mutant, rps5, which contains a mutation in the chloroplast ribosomal protein RPS5 and has reduced chloroplast translation, also displayed decreased sensitivity to Pi starvation. Furthermore, wild type plants treated with lincomycin, a chemical inhibitor of chloroplast translation, showed similar growth phenotypes and Pi starvation responses as dps1 and rps5. These results suggest that impaired chloroplast translation desensitizes plants to Pi starvation. Combined with previously published results showing that enhanced leaf photosynthesis augments plant responses to Pi starvation, we propose that the decrease in responses to Pi starvation in dps1, rps5, and lincomycin-treated plants is due to their reduced demand for Pi input from the environment.
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影响因子：
6.9
作者：
Niu Y;Chai R;Dong H;Wang H;Tang C;Zhang Y
通讯作者：
Zhang Y
影响因子：
6.9
作者：
Koiwa, H;Bressan, RA;Hasegawa, PM
通讯作者：
Hasegawa, PM
影响因子：
5.1
作者：
Li, Lihua;Liu, Chao;Lian, Xingming
通讯作者：
Lian, Xingming
影响因子：
2.6
作者：
Lloyd, JC;Zakhleniuk, OV;Raines, CA
通讯作者：
Raines, CA
DOI：
10.1073/pnas.0505266102
发表时间：
2005-08-16
影响因子：
11.1
作者：
Misson, J;Raghothama, KG;Thibaud, MC
通讯作者：
Thibaud, MC