Increased synovial lipodystrophy induced by high fat diet aggravates synovitis in experimental osteoarthritis.
Increased synovial lipodystrophy induced by high fat diet aggravates synovitis in experimental osteoarthritis.
复制标题
DOI：
10.1186/s13075-017-1473-z
复制
发表时间：
2017-12-01
影响因子：
4.9
通讯作者：
Largo R
中科院分区：
文献类型：
--
作者：
Larrañaga-Vera A;Lamuedra A;Pérez-Baos S;Prieto-Potin I;Peña L;Herrero-Beaumont G;Largo R
代谢综合征（MetS）可能与膝关节骨关节炎（OA）相关，但各组分与OA之间的相关性尚不清楚。我们的目的是研究高胆固醇血症对膝关节OA滑膜炎症的影响。实验分为两组，OA组（n = 10）和OA-HFD组（n = 10）。还监测了接受标准饮食（对照，n = 10）或喂食HFD（HFD，n = 6）的健康兔子。OA诱导后12周，分离滑膜并处理用于研究。喂食HFD的动物显示血清总胆固醇、甘油三酯和C-反应蛋白水平高于对照兔。OA诱导后12周，OA兔的滑膜炎症和巨噬细胞浸润增加，特别是在OA-HFD组。与OA组和对照组相比，OA-HFD组的滑膜脂肪组织面积、脂肪细胞大小和周脂蛋白-1A合成显著减少。HFD进一步增加OA滑膜中的促炎介质IL-1β、IL-6和TNF。然而，滑膜脂肪因子的基因表达，如瘦素和脂联素，在OA兔，特别是在OA-HFD组，与脂肪组织损失显着降低。然而，循环瘦素在HFD和OA-HFD组中上调。我们的研究结果表明，一个HFD是一个恶化的因素恶化滑膜炎症在OA，指导增加的巨噬细胞浸润和脂肪组织的去除，以及显着存在的促炎因子。滑膜脂肪细胞和血脂异常可能在MetS患者的OA关节恶化中发挥关键作用，支持肥胖和OA之间的联系超越机械负荷。本文的在线版本（doi：10.1186/s13075-017-1473-z）包含补充材料，可供授权用户使用。
Metabolic syndrome (MetS) may be associated with knee osteoarthritis (OA), but the association between the individual components and OA are not well-understood. We aimed to study the effect of hypercholesterolemia on synovial inflammation in knee OA. OA was surgically induced in rabbits fed with standard diet (OA group, n = 10) or in rabbits fed with high fat diet (OA-HFD, n = 10). Healthy rabbits receiving standard diet (Control, n = 10) or fed with HFD (HFD, n = 6) were also monitored. Twelve weeks after OA induction, synovial membranes were isolated and processed for studies. Animals fed HFD showed higher levels of total serum cholesterol, triglycerides and C-reactive protein than control rabbits. Twelve weeks after OA induction, synovial membrane inflammation and macrophage infiltration were increased in rabbits with OA, particularly in the OA-HFD group. Extensive decrease of synovial adipose tissue area, adipocyte size and perilipin-1A synthesis were observed in the OA-HFD group in comparison to the OA and control groups. The HFD further increased the proinflammatory mediators IL-1β, IL-6 and TNF in the OA synovium. However, the synovial gene expression of adipokines, such as leptin and adiponectin, were markedly decreased in the rabbits with OA, especially in the OA-HFD group, in correlation with adipose tissue loss. However, circulating leptin was upregulated in the HFD and OA-HFD groups. Our results indicate that a HFD is an aggravating factor worsening synovial membrane inflammation during OA, guided by increased infiltration of macrophages and removal of the adipose tissue, together with a remarkable presence of proinflammatory factors. Synovial adipocytes and dyslipemia could probably play pivotal roles in OA joint deterioration in patients with MetS, supporting that the link between obesity and OA transcends mechanical loading. The online version of this article (doi:10.1186/s13075-017-1473-z) contains supplementary material, which is available to authorized users.
登录
查看更多内容
DOI：
10.1152/ajpheart.00142.2009
发表时间：
2009-07-01
影响因子：
4.8
作者：
Largo, Raquel;Jose Martinez-Calatrava, Maria;Herrero-Beaumont, Gabriel
通讯作者：
Herrero-Beaumont, Gabriel
影响因子：
4.9
作者：
Ioan-Facsinay A;Kloppenburg M
通讯作者：
Kloppenburg M
影响因子：
7
作者：
Collins, K. H.;Reimer, R. A.;Herzog, W.
通讯作者：
Herzog, W.
影响因子：
7
作者：
de Munter, W.;van den Bosch, M. H.;van Lent, P. L.
通讯作者：
van Lent, P. L.
影响因子：
7
作者：
de Boer, T. N.;van Spil, W. E.;Mastbergen, S. C.
通讯作者：
Mastbergen, S. C.