The heterogeneous mechanical properties of adolescent growth plate cartilage: A study in rabbit.
The heterogeneous mechanical properties of adolescent growth plate cartilage: A study in rabbit.
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DOI：
10.1016/j.jmbbm.2022.105102
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发表时间：
2022-04
影响因子：
3.9
通讯作者：
Ferguson, Virginia L.
中科院分区：
文献类型：
--
作者：
Eckstein, Kevin N.;Thomas, Stacey M.;Scott, Adrienne K.;Neu, Corey P.;Hadley-Miller, Nancy A.;Payne, Karin A.;Ferguson, Virginia L.
生长板是一种软骨组织，其功能是延长儿童的骨骼。然而，当骨折时，生长板可能失去这一关键功能。我们对生长板断裂和力学生物学的理解目前受到生长板力学性能微尺度空间梯度信息稀疏的阻碍。在这项研究中，我们在9周龄新西兰大白兔（n = 15）胫骨近端生长板上进行了微压痕实验，利用线性弹性和非线性孔弹性材料模型来表征力学性能的空间变化。赫兹减少模量的平均压痕结果在380 ~ 690 kPa之间，在肥厚区上部有峰值，相邻区之间差异显著（p < 0.05）。利用这些动物的一个子集（n = 7），我们通过共聚焦荧光显微镜和拉曼光谱图谱表征了生长板的带状结构和细胞外基质含量。通过对生长板上基质含量与力学性能的比较，发现蛋白聚糖含量与压缩模量相关。这项研究是第一次测量生长板软骨微压痕的孔隙弹性力学性能，并辨别上下肥大带之间不同的力学性能。后一项发现可以解释典型生长板骨折的位置。我们报道的力学性能的空间变化强调了生长板的异质结构，这对未来再生种植体的设计和力学生物学模型具有重要意义。
The growth plate is a cartilaginous tissue that functions to lengthen bones in children. When fractured, however, the growth plate can lose this critical function. Our understanding of growth plate fracture and mechanobiology is currently hindered by sparse information on the growth plate’s microscale spatial gradients in mechanical properties. In this study, we performed microindentation across the proximal tibia growth plate of 9-week-old New Zealand White rabbits (n = 15) to characterize spatial variations in mechanical properties using linear elastic and nonlinear poroelastic material models. Mean indentation results for Hertz reduced modulus ranged from 380 to 690 kPa, with a peak in the upper hypertrophic zone and significant differences (p < 0.05) between neighboring zones. Using a subset of these animals (n = 7), we characterized zonal structure and extracellular matrix content of the growth plate through confocal fluorescent microscopy and Raman spectroscopy mapping. Comparison between mechanical properties and matrix content across the growth plate showed that proteoglycan content correlated with compressive modulus. This study is the first to measure poroelastic mechanical properties from microindentation across growth plate cartilage and to discern differing mechanical properties between the upper and lower hypertrophic zones. This latter finding may explain the location of typical growth plate fractures. The spatial variation in our reported mechanical properties emphasize the heterogeneous structure of the growth plate which is important to inform future regenerative implant design and mechanobiological models.
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影响因子：
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