Studies on cardiac myofibrillogenesis with antibodies to titin, actin, tropomyosin, and myosin
Studies on cardiac myofibrillogenesis with antibodies to titin, actin, tropomyosin, and myosin
复制标题
用肌联蛋白、肌动蛋白、原肌球蛋白和肌球蛋白抗体研究心肌原纤维形成
作者：
Seungho Wang;M. Greaser;E. Schultz;J. C. Bulinski;Jim;J.;James L Lessardll;S.
用肌联蛋白、肌动蛋白、原肌球蛋白和肌球蛋白的免疫荧光抗体在培养的鸡心肌细胞中检测心肌肌原纤维发生。原始心肌细胞最初含有应力纤维样结构（SFLS），α肌动蛋白和/或肌肉原肌球蛋白染色阳性。在某些情况下，肌肉原肌球蛋白和α肌动蛋白的染色是不成比例的，这表明这两种亚型的合成和/或组装成SFLS可能不是化学计量的。这些肌细胞中含有α肌动蛋白的SFLS可分为中央或外周;中央SFLS显示出正在发育的肌节肌联蛋白，而外周SFLS具有弱肌联蛋白荧光和更均匀的染色分布。肌联蛋白和肌球蛋白的肌节模式存在于这些结构上的多个位点。即使在两个或三个肌节阶段，一对肌联蛋白染色带也与每个发育中的A带明显相关，尽管偶尔注意到肌联蛋白带与半肌节相关的例子。肌节肌联蛋白模式的出现符合或先于α肌动蛋白或肌肉原肌球蛋白的肌节周期性。肌联蛋白在肌原纤维发生中的早期出现表明它可能在肌节组装过程中的细丝排列中起作用。
Cardiac myofibrillogenesis was examined in cultured chick cardiac cells by immunofluorescence using antibodies against titin, actin, tropomyosin, and myosin. Primitive cardiomyocytes initially contained stress fiber-like structures (SFLS) that stained positively for alpha actin and/or muscle tropomyosin. In some cases the staining for muscle tropomyosin and alpha actin was disproportionate; this suggests that the synthesis and/or assembly of these two isoforms into the SFLS may not be stoichiometric. The alpha actin containing SFLS in these myocytes could be classified as either central or peripheral; central SFLS showed developing sarcomeric titin while peripheral SFLS had weak titin fluorescence and a more uniform stain distribution. Sarcomeric patterns of titin and myosin were present at multiple sites on these structures. A pair of titin staining bands was clearly associated with each developing A band even at the two or three sarcomere stage, although occasional examples of a titin band being associated with a half sarcomere were noted. The appearance of sarcomeric titin patterns coincided or preceded sarcomere periodicity of either alpha actin or muscle tropomyosin. The early appearance of titin in myofibrillogenesis suggests it may have a role in filament alignment during sarcomere assembly.
登录
查看更多内容
横纹肌中的细胞骨架基质：肌联蛋白、星云蛋白和中间丝的作用。
影响因子：
--
作者：
Wang,K
通讯作者：
Wang,K
影响因子：
56.9
作者：
GILOH, H;SEDAT, JW
通讯作者：
SEDAT, JW
针对大鼠骨骼肌胚胎肌球蛋白重链的单克隆抗体。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gambke,B;Rubinstein,NA
通讯作者：
Rubinstein,NA
横纹肌中肌节相关的细胞骨架晶格。
DOI：
10.1007/978-1-4757-4723-2_10
发表时间：
1985
期刊：
Cell and muscle motility
影响因子：
--
作者：
Wang,K
通讯作者：
Wang,K
影响因子：
2.9
作者：
GIULIAN, GG;MOSS, RL;GREASER, M
通讯作者：
GREASER, M