Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis.
Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis.
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DOI：
10.3791/61616
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发表时间：
2020-08-21
影响因子：
1.2
通讯作者：
Dunnwald, Martine
中科院分区：
文献类型：
--
作者：
Rhea, Lindsey;Dunnwald, Martine
小鼠切除伤口模型已被广泛用于研究伤口愈合的每个顺序重叠阶段：炎症，增殖和重塑。鼠类伤口具有组织学上明确定义且易于识别的伤口床，在伤口床上，愈合过程的这些不同阶段是可测量的。在该领域内，通常使用任意定义的伤口“中间”进行组织学分析。然而，伤口是一个三维实体，通常不是组织学对称的，支持需要一个明确的和强大的量化方法来检测具有小效应大小的形态学缺陷。在本协议中，我们描述了在小鼠中创建双侧全层切除伤口的程序，以及如何使用图像处理程序在选择的连续切片上测量形态测量参数的详细说明。伤口长度、表皮长度、表皮面积和伤口面积的二维测量与切片之间的已知距离结合使用，以外推覆盖伤口的三维表皮面积、总伤口面积、表皮体积和伤口体积。虽然这种详细的组织学分析比常规分析消耗更多的时间和资源，但其严格性增加了在固有复杂的伤口愈合过程中检测新表型的可能性。
The murine excisional wound model has been used extensively to study each of the sequentially overlapping phases of wound healing: inflammation, proliferation and remodeling. Murine wounds have a histologically well-defined and easily recognizable wound bed over which these different phases of the healing process are measurable. Within the field, it is common to use an arbitrarily defined “middle” of the wound for histological analyses. However, wounds are a three-dimensional entity and often not histologically symmetrical, supporting the need for a well-defined and robust method of quantification to detect morphometric defects with a small effect size. In this protocol, we describe the procedure for creating bilateral, full-thickness excisional wounds in mice as well as a detailed instruction on how to measure morphometric parameters using an image processing program on select serial sections. The two-dimension measurements of wound length, epidermal length, epidermal area, and wound area are used in combination with the known distance between sections to extrapolate the three-dimension epidermal area covering the wound, overall wound area, epidermal volume and wound volume. Although this detailed histological analysis is more time and resource consuming than conventional analyses, its rigor increases the likelihood of detecting novel phenotypes in an inherently complex wound healing process.
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影响因子：
17.1
作者：
Eming SA;Martin P;Tomic-Canic M
通讯作者：
Tomic-Canic M
影响因子：
2.5
作者：
Rhea, Lindsey;Canady, Franklin J.;Le, Marc;Reeb, Tanner;Canady, John W.;Kacmarynski, Deborah S. F.;Avvari, Rishika;Biggs, Leah C.;Dunnwald, Martine
通讯作者：
Dunnwald, Martine
DOI：
10.1111/wrr.12148
发表时间：
2014-03
期刊：
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
影响因子：
--
作者：
Ansell DM;Campbell L;Thomason HA;Brass A;Hardman MJ
通讯作者：
Hardman MJ
影响因子：
2.9
作者：
Colwell, AS;Krummel, TM;Lorenz, HP
通讯作者：
Lorenz, HP
影响因子：
2.9
作者：
Gerharz, Michael;Baranowsky, Ankev;Wickenhauser, Claudia
通讯作者：
Wickenhauser, Claudia