Antimicrobial peptide-like genes in Nasonia vitripennis: a genomic perspective.
Antimicrobial peptide-like genes in Nasonia vitripennis: a genomic perspective.
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Nasonia vitripennis 中的抗菌肽样基因：基因组视角
DOI：
10.1186/1471-2164-11-187
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发表时间：
2010-03-19
期刊：
影响因子：
4.4
通讯作者：
Zhu S
中科院分区：
文献类型：
--
作者：
Tian C;Gao B;Fang Q;Ye G;Zhu S
背景
抗菌肽（Antimicrobial peptides，AMP）是天然免疫系统的重要组成部分，能对多种病原微生物迅速产生应答。昆虫作为抗菌肽的丰富来源，在了解免疫系统的基础生物学和寻找抗感染药物设计的分子模板方面引起了科学家的极大关注。尽管已经从不同的昆虫物种中鉴定了大量的AMP，但是从寄生昆虫中获得的关于这些肽的信息很少。
结果
通过利用集成计算方法系统挖掘小菜蛾基因组，我们建立了第一个AMP库，其成员具有广泛的序列和结构多样性，可以区分为多种分子类型，包括具有半胱氨酸稳定的α-螺旋和β-折叠（CSαβ）折叠的昆虫和真菌防御素样肽（DLP）、富含Pro或Gly的abaecins和hyperetaecins、富含Gly的bacins和glycins、富含Glycins的bacins和glycins、富含Glycins的bacins和glycins。具有抑制剂胱氨酸结（ICK）折叠的鲎tachystatin型AMP;和线性α-螺旋肽。7个N.验证了丽翅虫AMP基因，合成了两种代表性的抗菌肽，并对其进行了功能鉴定。与西方蜜蜂（Apis mellifera）和几种非蜂目模式昆虫比较，发现北方蜜蜂（N.丽翅虫进化出一种复杂的抗菌免疫系统，具有更多的基因和更大的蛋白质前体。已经认识到可能导致复杂性增加的三种经典策略：1）基因复制; 2）外显子复制;和3）外显子改组。
结论
本研究建立了N.通过使用计算和实验相结合的策略，研究了与抗微生物免疫相关的丽翅虫肽组。作为寄生蜂的第一个AMP库，我们的研究结果为进一步研究这些新发现的AMP样基因在这类昆虫中的免疫学和进化意义提供了基础平台。
Background
Antimicrobial peptides (AMPs) are an essential component of innate immunity which can rapidly respond to diverse microbial pathogens. Insects, as a rich source of AMPs, attract great attention of scientists in both understanding of the basic biology of the immune system and searching molecular templates for anti-infective drug design. Despite a large number of AMPs have been identified from different insect species, little information in terms of these peptides is available from parasitic insects.
Results
By using integrated computational approaches to systemically mining the Hymenopteran parasitic wasp Nasonia vitripennis genome, we establish the first AMP repertoire whose members exhibit extensive sequence and structural diversity and can be distinguished into multiple molecular types, including insect and fungal defensin-like peptides (DLPs) with the cysteine-stabilized α-helical and β-sheet (CSαβ) fold; Pro- or Gly-rich abaecins and hymenoptaecins; horseshoe crab tachystatin-type AMPs with the inhibitor cystine knot (ICK) fold; and a linear α-helical peptide. Inducible expression pattern of seven N. vitripennis AMP genes were verified, and two representative peptides were synthesized and functionally identified to be antibacterial. In comparison with Apis mellifera (Hymenoptera) and several non-Hymenopteran model insects, N. vitripennis has evolved a complex antimicrobial immune system with more genes and larger protein precursors. Three classical strategies that are likely responsible for the complexity increase have been recognized: 1) Gene duplication; 2) Exon duplication; and 3) Exon-shuffling.
Conclusion
The present study established the N. vitripennis peptidome associated with antimicrobial immunity by using a combined computational and experimental strategy. As the first AMP repertoire of a parasitic wasp, our results offer a basic platform for further studying the immunological and evolutionary significances of these newly discovered AMP-like genes in this class of insects.
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DOI：
10.1016/j.cbpb.2008.02.007
发表时间：
2008-06-01
影响因子：
2.2
作者：
Choi, Yong Soo;Choo, Young Moo;Jin, Byung Rae
通讯作者：
Jin, Byung Rae
使用活体染色方法筛选和克隆抗菌 DNA 序列
影响因子：
3.5
作者：
Cheng, Xiongying;Liu, Guozhen;Altosaar, Illimar
通讯作者：
Altosaar, Illimar
影响因子：
10.7
作者：
Ding, KY;Zhou, K;Shen, Y
通讯作者：
Shen, Y
影响因子：
8
作者：
de la Vega, RCR;García, BI;Possani, LD
通讯作者：
Possani, LD
影响因子：
56.9
作者：
Christophides, GK;Zdobnov, E;Kafatos, FC
通讯作者：
Kafatos, FC