Transgenerational Effects of Prenatal Endocrine Disruption on Reproductive and Sociosexual Behaviors in Sprague Dawley Male and Female Rats.
Transgenerational Effects of Prenatal Endocrine Disruption on Reproductive and Sociosexual Behaviors in Sprague Dawley Male and Female Rats.
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DOI：
10.3390/toxics10020047
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发表时间：
2022-01-20
期刊：
影响因子：
4.6
通讯作者：
Gore AC
中科院分区：
文献类型：
--
作者：
Kermath BA;Thompson LM;Jefferson JR;Ward MHB;Gore AC
内分泌干扰化学品（EDCs）导致内分泌和神经行为的变化，特别是由于在怀孕和生命早期的发育暴露。此外，生殖系暴露（F2）和表观遗传生殖系传递（F3）世代可能分别诱导代际和跨代表型变化。在这里，我们评估了产前Aroclor 1221（A1221），轻度氯化混合的多氯联苯已知具有弱雌激素作用机制的生殖和社会性行为的结果;苯甲酸雌二醇（EB），阳性对照;或车辆（3%DMSO的芝麻油）在F1，F2和F3代雄性和雌性大鼠。在胚胎第16天和第18天给予EDCs治疗，并监测F1后代的发育和成年行为。通过与未处理大鼠交配产生F2后代，在成年期进行F2的表型分析，并以类似方式产生F3代并进行表型分析。尽管未发现给药对F1或F3代发育和生理学的影响，但在F2代中，A1221增加了雄性动物的体重和雌性动物的子宫重量。F1和F2代的交配行为结果表明，F1 A1221雌性动物具有较长的前凸潜伏期。在雄性动物中，EB组F2代的贴装频率降低。在F3代，男性的超声波发声的数量减少EB，EB和A1221时，性别相结合。最后，在F3代的伴侣偏好测试显示，天真的女性更喜欢F3-EB超过未经处理的男性，而天真的男性更喜欢未经处理的F3-EB或F3-A1221男性。总的来说，这些结果表明，由于直接或祖先的EDC暴露，每一代都有独特的性别特异性行为表型。
Endocrine-disrupting chemicals (EDCs) lead to endocrine and neurobehavioral changes, particularly due to developmental exposures during gestation and early life. Moreover, intergenerational and transgenerational phenotypic changes may be induced by germline exposure (F2) and epigenetic germline transmission (F3) generation, respectively. Here, we assessed reproductive and sociosexual behavioral outcomes of prenatal Aroclor 1221 (A1221), a lightly chlorinated mix of PCBs known to have weakly estrogenic mechanisms of action; estradiol benzoate (EB), a positive control; or vehicle (3% DMSO in sesame oil) in F1-, F2-, and F3-generation male and female rats. Treatment with EDCs was given on embryonic day (E) 16 and 18, and F1 offspring monitored for development and adult behavior. F2 offspring were generated by breeding with untreated rats, phenotyping of F2s was performed in adulthood, and the F3 generation were similarly produced and phenotyped. Although no effects of treatment were found on F1 or F3 development and physiology, in the F2 generation, body weight in males and uterine weight in females were increased by A1221. Mating behavior results in F1 and F2 generations showed that F1 A1221 females had a longer latency to lordosis. In males, the F2 generation showed decreased mount frequency in the EB group. In the F3 generation, numbers of ultrasonic vocalizations were decreased by EB in males, and by EB and A1221 when the sexes were combined. Finally, partner preference tests in the F3 generation revealed that naïve females preferred F3-EB over untreated males, and that naïve males preferred untreated over F3-EB or F3-A1221 males. As a whole, these results show that each generation has a unique, sex-specific behavioral phenotype due to direct or ancestral EDC exposure.
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DOI：
10.1073/pnas.0610410104
发表时间：
2007-04-03
影响因子：
11.1
作者：
Crews, David;Gore, Andrea C.;Skinner, Michael K.
通讯作者：
Skinner, Michael K.
DOI：
10.1111/j.1749-6632.1986.tb27996.x
发表时间：
1986-12-31
影响因子：
5.2
作者：
BARFIELD, RJ;THOMAS, DA
通讯作者：
THOMAS, DA
影响因子：
3.5
作者：
Gillette R;Reilly MP;Topper VY;Thompson LM;Crews D;Gore AC
通讯作者：
Gore AC
影响因子：
3.3
作者：
Brudzynski SM
通讯作者：
Brudzynski SM
影响因子：
3.5
作者：
Henley, C. L.;Nunez, A. A.;Clemens, L. G.
通讯作者：
Clemens, L. G.