Digital Assembly of Spherical Viscoelastic Bio‐Ink Particles
Digital Assembly of Spherical Viscoelastic Bio‐Ink Particles
复制标题
球形粘弹性生物墨水颗粒的数字组装
DOI：
10.1002/adfm.202109004
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发表时间：
2021
影响因子：
19
通讯作者：
Oberholzer, Jose
中科院分区：
文献类型：
--
作者：
Zhu, Jinchang;He, Yi;Kong, Linlin;He, Zhijian;Kang, Kaylen Y.;Grady, Shannon P.;Nguyen, Leander Q.;Chen, Dong;Wang, Yong;Oberholzer, Jose
3D生物打印添加组装生物墨水以制造组织模仿生物构造，但典型的构建块仅限于1D细丝。在这里，它是开发了一种体素化生物打印技术的数字组装的球形颗粒（DASP），这是有效的0D体素的3D结构的基本单元。示出了DASP使得能够按需生成、沉积和组装粘弹性生物墨水液滴。开发了双参数图以概述打印良好保真度的球形颗粒所需的生物油墨的粘弹性。此外，还开发了一种策略，用于工程生物油墨，使其具有独立可控的粘弹性和网格尺寸，这是生物材料的两个最重要但内在耦合的物理特性。使用DASP，创建了由互连但可区分的水凝胶颗粒组成的机械坚固的多尺度多孔支架。最后，展示了该支架在包封人胰岛以实现持续响应性胰岛素释放中的应用。结合生物墨水设计的知识，DASP可用于设计高度异质但组织紧密的组织结构，以用于治疗应用。
3D bioprinting additively assembles bio‐inks to manufacture tissue‐mimicking biological constructs, but with the typical building blocks limited to 1D filaments. Here, it is developed a voxelated bioprinting technique for the digital assembly of spherical particles (DASP), which are effectively 0D voxels—the basic unit of 3D structures. It is shown that DASP enables on‐demand generation, deposition, and assembly of viscoelastic bio‐ink droplets. A two‐parameter diagram is developed to outline the viscoelasticity of bio‐inks required for printing spherical particles of good fidelity. Moreover, a strategy is developed for engineering bio‐inks with independently controllable viscoelasticity and mesh size, two of the most important yet intrinsically coupled physical properties of biomaterials. Using DASP, mechanically robust, multiscale porous scaffolds composed of interconnected yet distinguishable hydrogel particles are created. Finally, it is demonstrated the application of the scaffolds in encapsulating human pancreatic islets for sustained responsive insulin release. Together with the knowledge of bio‐ink design, DASP might be used to engineer highly heterogeneous, yet tightly organized tissue constructs for therapeutic applications.
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