Molecular characterization of mucosal adherent bacteria and associations with colorectal adenomas.
Molecular characterization of mucosal adherent bacteria and associations with colorectal adenomas.
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DOI：
10.4161/gmic.1.3.12360
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发表时间：
2010-05
期刊：
影响因子：
12.2
通讯作者：
Keku TO
中科院分区：
文献类型：
--
作者：
Shen XJ;Rawls JF;Randall T;Burcal L;Mpande CN;Jenkins N;Jovov B;Abdo Z;Sandler RS;Keku TO
人类大肠由复杂多样的细菌群落定殖。然而，肠道细菌和腺瘤（结直肠癌前体）之间的关系尚不清楚。本研究旨在描述正常结肠中粘附细菌的特征，并评估与结直肠腺瘤相关的群落组成的差异。我们评估了21例腺瘤和23例非腺瘤受试者的正常结肠粘膜中的粘附细菌。末端限制性片段长度多态性，克隆测序和荧光原位杂交分析的16S rRNA基因被用来表征粘附细菌。共335个克隆进行测序和系统发育和分类学分析。通过UniFrac和相似性矩阵分析评价病例和对照之间细菌组成的差异。总体而言，厚壁菌门（62%）、拟杆菌门（26%）和变形菌门（11%）是最占优势的门。细菌组成在病例和对照之间存在显著差异（UniFrac p < 0.001）。与对照组相比，我们观察到病例中变形菌门的丰度显着较高（p < 0.05），拟杆菌门的丰度较低（p < 0.05）。在属的水平上，病例显示多雷亚属的丰度增加。（p < 0.005）、粪杆菌属（Faecalibacterium spp.）（p < 0.05）和类杆菌属的较低比例。（p < 0.03）和粪球菌属（Coprococcus spp.）（p < 0.05）。病例组细菌多样性和丰富度高于对照组。这些发现表明，腺瘤相关的细菌群落组成的改变可能有助于结直肠癌的病因。这些发现的扩展可能导致操纵微生物群以预防结直肠腺瘤和癌症以及识别高风险个体的策略。
The human large bowel is colonized by complex and diverse bacterial communities. However, the relationship between commensal bowel bacteria and adenomas (colorectal cancer precursors) is unclear. This study aimed to characterize adherent bacteria in normal colon and evaluate differences in community composition associated with colorectal adenomas. We evaluated adherent bacteria in normal colonic mucosa of 21 adenoma and 23 non-adenoma subjects enrolled in a cross sectional study. Terminal restriction fragment length polymorphism, clone sequencing and fluorescent in-situ hybridization analysis of the 16S rRNA genes were used to characterize adherent bacteria. A total of 335 clones were sequenced and processed for phylogenetic and taxonomic analysis. Differences in bacterial composition between cases and controls were evaluated by UniFrac and analysis of similarity matrix. Overall, Firmicutes (62%), Bacteroidetes (26%) and Proteobacteria (11%) were the most dominant phyla. The bacterial composition differed significantly between cases and controls (UniFrac p < 0.001). We observed significantly higher abundance of Proteobacteria (p < 0.05) and lower abundance of Bacteroidetes (p < 0.05) in cases compared to controls. At the genus level, case subjects showed increased abundance of Dorea spp. (p < 0.005), Faecalibacterium spp. (p < 0.05) and lower proportions of Bacteroides spp. (p < 0.03) and Coprococcus spp. (p < 0.05) than controls. Cases had higher bacterial diversity and richness than controls. These findings reveal that alterations in bacterial community composition associated with adenomas may contribute to the etiology of colorectal cancer. Extension of these findings could lead to strategies to manipulate the microbiota to prevent colorectal adenomas and cancer as well as to identify individuals at high risk.
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