Rhizosphere suppression hinders antibiotic resistance gene (ARG) spread under bacterial invasion.
Rhizosphere suppression hinders antibiotic resistance gene (ARG) spread under bacterial invasion.
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DOI：
10.1016/j.onehlt.2023.100481
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发表时间：
2023-06
期刊：
影响因子：
5
通讯作者：
Salles, Joana Falcao
中科院分区：
文献类型：
--
作者：
Li, Yuchan;Deng, Xuhui;Zhang, Na;Shen, Zongzhuan;Li, Rong;Shen, Qirong;Salles, Joana Falcao
根际是“同一个健康”场景中极其重要的组成部分，它连接着土壤微生物组和植物，其中抗生素抗性基因（ARG）的潜在富集可能最终流入人类食物链。尽管土传疾病的发生率增加，导致农药和抗生素生物防治剂的使用增加，但对 ARG 在根际传播动态的了解在很大程度上被忽视。在这里，选择在有利于和抑制病原体青枯菌的土壤中生长的番茄幼苗作为模型，研究有或没有病原体入侵的情况下 ARG 在根际的传播。宏基因组数据显示，青枯菌入侵增加了 ARG 和移动遗传元件 (MGE) 的密度。尽管我们在两种土壤中都发现了源自人类致病菌的 ARG，但在抑制性土壤中富集程度有所减轻。总之，抑制性土壤通过抑制病原体阻碍了 ARG 的传播，并且具有抗生素抗性的类群数量较少。细菌病原体入侵增加了根际内 ARG 和 MGE 的密度。富集的ARGs源自入侵病原体和人类致病菌。抑制性根际土壤可以减轻 ARGs 的富集。携带 ARG 的入侵和本地类群数量减少导致了缓解。
The rhizosphere is an extremely important component of the “one health” scenario by linking the soil microbiome and plants, in which the potential enrichment of antibiotic resistance genes (ARGs) might ultimately flow into the human food chain. Despite the increased occurrence of soil-borne diseases, which can lead to increased use of pesticides and antibiotic-producing biocontrol agents, the understanding of the dynamics of ARG spread in the rhizosphere is largely overlooked. Here, tomato seedlings grown in soils conducive and suppressive to the pathogen Ralstonia solanacearum were selected as a model to investigate ARG spread in the rhizosphere with and without pathogen invasion. Metagenomics data revealed that R. solanacearum invasion increased the density of ARGs and mobile genetic elements (MGEs). Although we found ARGs originating from human pathogenic bacteria in both soils, the enrichment was alleviated in the suppressive soil. In summary, the suppressive soil hindered ARG spread through pathogen suppression and had a lower number of taxa carrying antibiotic resistance. Bacterial pathogen invasion increased the density of ARGs and MGEs within rhizosphere. Enriched ARGs were originated from the Invading pathogen and human pathogenic bacteria. Suppressive rhizosphere soil could alleviate the ARGs enrichment. Lower number of invaded and native taxa carrying ARGs lead to the alleviation.
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影响因子：
11
作者：
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通讯作者：
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影响因子：
5.1
作者：
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通讯作者：
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影响因子：
3.6
作者：
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影响因子：
11.4
作者：
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通讯作者：
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由于连续花生单一栽培，真菌病原体的积累以植物有益真菌为代价
影响因子：
9.7
作者：
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通讯作者：
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