Isolation and identification of Bifidobacterium species from feces of captive chimpanzees.
Isolation and identification of Bifidobacterium species from feces of captive chimpanzees.
复制标题
DOI：
10.12938/bmfh.16-027
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Osawa R
中科院分区：
文献类型：
--
作者：
Nomoto R;Takano S;Tanaka K;Tsujikawa Y;Kusunoki H;Osawa R
最近，人们经常研究来自黑猩猩肠道的双歧杆菌，它们在系统发育上与人类接近，并且具有与人类相似的摄食习惯。鉴于此，我们推测黑猩猩和人类一样，也拥有独特的双歧杆菌多样性。我们在此描述了从圈养黑猩猩粪便样本中分离出的双歧杆菌分类学新成员。通过定量聚合酶链反应在所有粪便样本中检测到双歧杆菌。一种类似假长双歧杆菌的物种在五只黑猩猩的粪便中占主导地位，但使用针对 groEL 基因序列的假长双歧杆菌特异性引物无法检测到该物种。从这组五只黑猩猩中分离出七株双歧杆菌，所有分离株均被鉴定为假长双歧杆菌。以前经常从非灵长类动物和人类中分离出假长双歧杆菌；然而，在这里我们证明了它在非人类灵长类动物中的存在。
Recently, gut-dwelling bifidobacteria from chimpanzees, which are phylogenetically close to humans and have feeding habits similar to humans, have been frequently investigated. Given this, we speculated that like humans, chimpanzees would have a unique diversity of bifidobacteria. We herein describe a taxonomically novel member of bifidobacteria isolated from fecal samples of captive chimpanzees. Bifidobacteria were detected in all fecal samples by quantitative polymerase chain reaction. A Bifidobacterium pseudolongum-like species, which could not be detected using B. pseudolongum-specific primers targeting the groEL gene sequence, was dominant in the feces of five chimpanzees. Seven bifidobacterial strains were isolated from this group of five chimpanzees, and all isolates were identified as B. pseudolongum. B. pseudolongum has previously often been isolated from non-primate animals as well as humans; however, here we demonstrate its presence in a nonhuman primate species.
登录
查看更多内容
DOI：
10.12938/bmfh.33.99
发表时间：
2014-01-01
期刊：
Bioscience of microbiota, food and health
影响因子：
--
作者：
Mitsuoka, Tomotari
通讯作者：
Mitsuoka, Tomotari
影响因子：
2.4
作者：
Uenishi, Gentaro;Fujita, Shiho;Ushida, Kazunari
通讯作者：
Ushida, Kazunari
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M
影响因子：
4.4
作者：
Matsuki, T;Watanabe, K;Tanaka, R
通讯作者：
Tanaka, R
影响因子：
3.7
作者：
Makino H;Kushiro A;Ishikawa E;Kubota H;Gawad A;Sakai T;Oishi K;Martin R;Ben-Amor K;Knol J;Tanaka R
通讯作者：
Tanaka R