Mother-to-infant transmission of intestinal bifidobacterial strains has an impact on the early development of vaginally delivered infant's microbiota.
Mother-to-infant transmission of intestinal bifidobacterial strains has an impact on the early development of vaginally delivered infant's microbiota.
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DOI：
10.1371/journal.pone.0078331
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tanaka R
中科院分区：
文献类型：
--
作者：
Makino H;Kushiro A;Ishikawa E;Kubota H;Gawad A;Sakai T;Oishi K;Martin R;Ben-Amor K;Knol J;Tanaka R
双歧杆菌是婴儿肠道微生物群的主要组成部分之一。研究表明，婴儿早期双歧杆菌的定植对于以后的健康非常重要。然而，有关这些微生物来源的信息仍然有限。在这里，我们研究了母体肠道菌群中特定的双歧杆菌菌株是否会传播到婴儿的肠道。粪便样本采集自 17 对健康母婴（阴道分娩：12 对；剖腹产分娩：5 对）。产前两次收集母亲的粪便。婴儿出生后0(胎便)、3、7、30、90天时收集粪便。通过多位点测序分型对从粪便中分离的双歧杆菌进行基因分型，并通过实时定量PCR分析婴儿粪便中双歧杆菌计数的变化。属于青春双歧杆菌、两歧双歧杆菌、链状双歧杆菌、长双歧杆菌亚种的染色剂。经鉴定，长链双歧杆菌和假链状双歧杆菌在母亲和婴儿的肠道之间是单系的。 12 名阴道分娩婴儿中有 11 名携带至少一种单系菌株。单系菌株所属物种的双歧杆菌计数在出生后 3 天内主要在婴儿肠道中增加。在剖腹产婴儿中，未观察到单系菌株。此外，直到7天大时，双歧杆菌计数均显着低于阴道分娩的婴儿。在阴道出生的婴儿中，一些双歧杆菌菌株从母亲传播并在出生后不久在婴儿的肠道中定殖。我们的数据表明，母亲的肠道是阴道分娩婴儿肠道微生物群的重要来源。
Bifidobacterium species are one of the major components of the infant's intestine microbiota. Colonization with bifidobacteria in early infancy is suggested to be important for health in later life. However, information remains limited regarding the source of these microbes. Here, we investigated whether specific strains of bifidobacteria in the maternal intestinal flora are transmitted to their infant's intestine. Fecal samples were collected from healthy 17 mother and infant pairs (Vaginal delivery: 12; Cesarean section delivery: 5). Mother's feces were collected twice before delivery. Infant's feces were collected at 0 (meconium), 3, 7, 30, 90 days after birth. Bifidobacteria isolated from feces were genotyped by multilocus sequencing typing, and the transitions of bifidobacteria counts in infant's feces were analyzed by quantitative real-time PCR. Stains belonging to Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium catenulatum, Bifidobacterium longum subsp. longum, and Bifidobacterium pseudocatenulatum, were identified to be monophyletic between mother's and infant's intestine. Eleven out of 12 vaginal delivered infants carried at least one monophyletic strain. The bifidobacterial counts of the species to which the monophyletic strains belong, increased predominantly in the infant's intestine within 3 days after birth. Among infants delivered by C-section, monophyletic strains were not observed. Moreover, the bifidobacterial counts were significantly lower than the vaginal delivered infants until 7 days of age. Among infants born vaginally, several Bifidobacterium strains transmit from the mother and colonize the infant's intestine shortly after birth. Our data suggest that the mother's intestine is an important source for the vaginal delivered infant's intestinal microbiota.
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影响因子：
10.4
作者：
Chen H;Quandt SA;Grzywacz JG;Arcury TA
通讯作者：
Arcury TA
影响因子：
4.4
作者：
Lundell, Anna-Carin;Bjornsson, Viktor;Rudin, Anna
通讯作者：
Rudin, Anna
影响因子：
2.5
作者：
Huurre, Anu;Kalliomaki, Marko;Isolauri, Erika
通讯作者：
Isolauri, Erika
影响因子：
2.6
作者：
Jimenez, Esther;Marin, Maria L.;Rodriguez, Juan M.
通讯作者：
Rodriguez, Juan M.
影响因子：
3.7
作者：
El Aila, Nabil Abdullah;Tency, Inge;Vaneechoutte, Mario
通讯作者：
Vaneechoutte, Mario