Complete genome sequence and recombination analysis of infectious bronchitis virus attenuated vaccine strain H120.
Complete genome sequence and recombination analysis of infectious bronchitis virus attenuated vaccine strain H120.
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传染性支气管炎病毒弱毒疫苗株H120全基因组序列及重组分析
DOI：
10.1007/s11262-010-0517-0
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发表时间：
2010-12
期刊：
影响因子：
1.6
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Zhang Y;Wang HN;Wang T;Fan WQ;Zhang AY;Wei K;Tian GB;Yang X
传染性支气管炎病毒(IBV)H120株是我国最早、最具代表性的传染性支气管炎活疫苗株之一。为研究H120的基因组特征，进一步了解其在IBV流行病学中的作用，对H120的全基因组进行了测序，并与其他IBV毒株进行了系统发育和重组分析。H120的全基因组全长为27631个核苷酸，结构与博德特毒株相似。我们发现菌株ZJ971可能是H120的一个毒力回复突变体。发现ZJ971有9个氨基酸突变和1个3个核苷酸缺失。此外，在包括H52、KQ6、SAIBK、Ark DPI 11和Ark DPI 101在内的5个IBV毒株中发现了与H120相关的潜在重组事件。这项研究表明，H120可能通过毒力逆转和重组导致新的IBV变异株的出现。
The strain H120 of infectious bronchitis virus (IBV) is one of the earliest and representative attenuated live Infectious Bronchitis vaccine strains. To investigate the genomic feature of H120 and further understand its role in the epidemiology of IBV, complete genome of H120 was sequenced and compared with sequences of other IBV strains by phylogenetic and recombination analysis. The complete genome of H120 is 27631 nucleotides in length and has a similar structure with that of Beaudette strain. We found that strain ZJ971 is probably a virulence revertant of H120. Nine amino acids changes and a three-nucleotide deletion were identified in ZJ971. Besides, potential recombination events associated with H120 were found in five IBV strains including H52, KQ6, SAIBK, Ark DPI 11, and Ark DPI 101. This study suggested that H120 might have contributed to the emergence of new IBV variants through both virulence reversion and recombination.
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