Comparative genetic and genomic analysis of the novel fusellovirus Sulfolobus spindle-shaped virus 10.
Comparative genetic and genomic analysis of the novel fusellovirus Sulfolobus spindle-shaped virus 10.
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DOI：
10.1093/ve/vey022
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发表时间：
2018-07
期刊：
影响因子：
5.3
通讯作者：
Stedman KM
中科院分区：
文献类型：
--
作者：
Goodman DA;Stedman KM
感染嗜热古生菌的病毒在结构和基因构成上都是独一无二的。柠檬形状的梭状病毒-感染硫叶目的成员，在80 °C和pH 3的条件下生长最适-是嗜热古细菌中最普遍和研究最好的病毒之一。尽管如此，关于这些病毒仍有许多需要了解的地方。为了研究梭状病毒的进化，我们分离并鉴定了一种新的梭形病毒--梭形病毒10(前称SSV-L1)。比较基因组分析突出了与SSV8和SSV9的显著相似性，以及梭状病毒科内的启动子元件的保守性。SSV10编码5个在梭状病毒科内外没有同源性的ORF，以及一个功能类似Cas4的ORF，它可能在逃避CRISPR介导的宿主防御中发挥作用。此外，我们还证明了SSV10以与SSV1的突变一致的方式抵抗突变的能力。
Viruses that infect thermophilic Archaea are unique in both their structure and genetic makeup. The lemon-shaped fuselloviruses—which infect members of the order Sulfolobales, growing optimally at 80 °C and pH 3—are some of the most ubiquitous and best studied viruses of the thermoacidophilic Archaea. Nonetheless, much remains to be learned about these viruses. In order to investigate fusellovirus evolution, we have isolated and characterized a novel fusellovirus, Sulfolobus spindle-shaped virus 10 (formerly SSV-L1). Comparative genomic analyses highlight significant similarity with both SSV8 and SSV9, as well as conservation of promoter elements within the Fuselloviridae. SSV10 encodes five ORFs with no homology within or outside of the Fuselloviridae, as well as a putatively functional Cas4-like ORF, which may play a role in evading CRISPR-mediated host defenses. Moreover, we demonstrate the ability of SSV10 to withstand mutation in a fashion consistent with mutagenesis in SSV1.
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