Fabrication and maturation of integrated biphasic anatomic mesenchymal stromal cell-laden composite scaffolds for osteochondral repair and joint resurfacing.
Fabrication and maturation of integrated biphasic anatomic mesenchymal stromal cell-laden composite scaffolds for osteochondral repair and joint resurfacing.
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DOI：
10.1002/jor.24969
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发表时间：
2021-11
期刊：
影响因子：
--
通讯作者：
Mauck RL
中科院分区：
文献类型：
--
作者：
Fryhofer GW;Zlotnick HM;Stoeckl BD;Farrell MJ;Steinberg DR;Mauck RL
关节软骨损伤可导致关节广泛侵蚀和骨关节炎的早期发作。为了解决这个问题，我们最近开发了一种快速制造方法来生产患者特定的工程软骨组织，以取代整个关节面。在这里，我们通过将载有间充质基质细胞的水凝胶（甲基丙烯酸酯化透明质酸，MeHA）与多孔聚己内酯（PCL）骨整合相结合来扩展这项工作，并评估这些结构随时间推移的组成和机械性能。为了改善初始构建体稳定性，首先通过在水凝胶输注之前改变PCL预处理（在乙醇之前用氢氧化钠）来优化PCL/水凝胶界面参数。接下来，形成圆柱形骨软骨构建体，并在含有TGFβ3的培养基中培养长达8周，评价构建体的活力、组织学特征和生化含量。机械性能也进行了评估，在轴向压缩，并通过界面剪切强度测定。结果表明，制造过程与细胞活力相容，并且构建物的生物化学含量和机械性能随时间增加。有趣的是，压缩性能在5周时达到峰值，而界面剪切性能在该时间点之后继续改善。最后，这些制造方法与根据肢体特异性CT成像数据开发的定制模具相结合，以创建解剖学上可植入的细胞接种生物关节表面，其显示出与骨软骨圆柱体相似的成熟。未来的工作将在关键尺寸的骨软骨修复和整个关节表面置换的大型动物模型中应用这些进展。
Articular cartilage injury can lead to joint-wide erosion and the early onset of osteoarthritis. To address this, we recently developed a rapid fabrication method to produce patient-specific engineered cartilage tissues to replace an entire articular surface. Here, we extended that work by coupling a mesenchymal stromal cell-laden hydrogel (methacrylated hyaluronic acid, MeHA) with the porous polycaprolactone (PCL) bone integrating phase and assessed the composition and mechanical performance of these constructs over time. To improve initial construct stability, PCL/hydrogel interface parameters were first optimized by varying PCL pre-treatment (with sodium hydroxide before ethanol) prior to hydrogel infusion. Next, cylindrical osteochondral constructs were formed and cultured in media containing TGFβ3 for up to 8 weeks, with constructs evaluated for viability, histological features, and biochemical content. Mechanical properties were also assessed in axial compression and via an interface shear strength assay. Results showed that the fabrication process was compatible with cell viability, and that construct biochemical content and mechanical properties increased with time. Interestingly, compressive properties peaked at five weeks, while interfacial shear properties continued to improve beyond this time point. Lastly, these fabrication methods combined with a custom mold developed from limb-specific CT imaging data to create an anatomic implantable cell-seeded biologic joint surface, which showed similar maturation as the osteochondral cylinders. Future work will apply these advances in large animal models of critically-sized osteochondral repair and whole joint resurfacing.
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影响因子：
7
作者：
Bian L;Angione SL;Ng KW;Lima EG;Williams DY;Mao DQ;Ateshian GA;Hung CT
通讯作者：
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影响因子：
46.9
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DOI：
10.1007/s10856-019-6218-x
发表时间：
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影响因子：
3.7
作者：
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通讯作者：
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影响因子：
9.7
作者：
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通讯作者：
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