Distal and proximal actions of peptide pheromone M-factor control different conjugation steps in fission yeast.
Distal and proximal actions of peptide pheromone M-factor control different conjugation steps in fission yeast.
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DOI：
10.1371/journal.pone.0069491
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Shimoda C
中科院分区：
文献类型：
--
作者：
Seike T;Nakamura T;Shimoda C
在裂糖酵母中，p型单倍体细胞（P-cells）和m型单倍体细胞（M-cells）之间的结合离不开交配信息素信号的传递。由m细胞产生的肽信息素m因子被p细胞的受体识别。一个没有m因子的突变体，其中m因子编码基因被删除，是完全不育的。在液体培养中，突变体未观察到性凝集，但通过添加外源合成m因子刺激p型特异性细胞粘附蛋白Map4的表达，可以恢复性凝集。然而，外源m因子未能恢复无m因子突变体的细胞融合缺陷。将无m因子的细胞加入到野生型P细胞和m细胞的混合物中，形成明显的细胞聚集体。值得注意的是，这些聚集物中也包含了无m因子的突变细胞。在这种混合培养中，p细胞优先与分泌m因子的m细胞结合，很少与不含m因子的m细胞结合。液体培养中交配参数的动力学表明，极化生长始于相对交配型细胞的接触区。综上所述，这些发现表明，低浓度的m因子诱导黏附素表达，导致最初的细胞-细胞黏附，这是一种“远端信息素作用”，但在黏附的p细胞附近直接分泌的m因子可能是一种“近端信息素作用”中细胞融合所必需的。
Mating pheromone signaling is essential for conjugation between haploid cells of P-type (P-cells) and haploid cells of M-type (M-cells) in Schizosaccharomyces pombe. A peptide pheromone, M-factor, produced by M-cells is recognized by the receptor of P-cells. An M-factor-less mutant, in which the M-factor-encoding genes are deleted, is completely sterile. In liquid culture, sexual agglutination was not observed in the mutant, but it could be recovered by adding exogenous synthetic M-factor, which stimulated expression of the P-type-specific cell adhesion protein, Map4. Exogenous M-factor, however, failed to recover the cell fusion defect in the M-factor-less mutant. When M-factor-less cells were added to a mixture of wild-type P- and M-cells, marked cell aggregates were formed. Notably, M-factor-less mutant cells were also incorporated in these aggregates. In this mixed culture, P-cells conjugated preferentially with M-cells secreting M-factor, and rarely with M-factor-less M-cells. The kinetics of mating parameters in liquid culture revealed that polarized growth commenced from the contact region of opposite mating-type cells. Taken together, these findings indicate that M-factor at a low concentration induces adhesin expression, leading to initial cell-cell adhesion in a type of “distal pheromone action”, but M-factor that is secreted directly in the proximity of the adhered P-cells may be necessary for cell fusion in a type of “proximal pheromone action”.
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影响因子：
11.4
作者：
KITAMURA, K;SHIMODA, C
通讯作者：
SHIMODA, C
DOI：
10.1016/j.bbrc.2005.10.156
发表时间：
2005-12-30
影响因子：
3.1
作者：
Kjærulff, S;Müller, S;Jensen, MR
通讯作者：
Jensen, MR
影响因子：
3.9
作者：
Ladds, G;Rasmussen, M;Davey, J
通讯作者：
Davey, J
影响因子：
10.5
作者：
IMAI, Y;YAMAMOTO, M
通讯作者：
YAMAMOTO, M
影响因子：
3.7
作者：
EGEL, R;EGELMITA.M
通讯作者：
EGELMITA.M