High-throughput linear optical stretcher for mechanical characterization of blood cells.
High-throughput linear optical stretcher for mechanical characterization of blood cells.
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DOI：
10.1002/cyto.a.22794
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发表时间：
2016-04
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本研究描述了一种线性光学拉伸器作为高通量机械性能细胞仪。定制的、便宜的和可扩展的光学器件将线性二极管条源成像到微流体通道中，其中细胞被流体动力学地聚焦到光学拉伸器中。在进入拉伸区域时，由紧密聚焦的激光在细胞膜处的折射产生的对映光学力使流动中的每个细胞变形。每个细胞在流出陷阱时都会松弛，并与拉伸状态进行比较以确定变形。将未处理的红细胞和中性粒细胞的变形反应与化学处理的细胞进行比较。在正常、二酰胺处理和戊二醛处理的红细胞之间以及正常和细胞松弛素D处理的中性粒细胞之间观察到统计学显著差异。基于纯的、未处理的红细胞和中性粒细胞群体的行为，测试了这些细胞的混合群体，并通过变形性鉴定了离散群体。
This study describes a linear optical stretcher as a high-throughput mechanical property cytometer. Custom, inexpensive, and scalable optics image a linear diode bar source into a microfluidic channel, where cells are hydrodynamically focused into the optical stretcher. Upon entering the stretching region, antipodal optical forces generated by the refraction of tightly focused laser light at the cell membrane deform each cell in flow. Each cell relaxes as it flows out of the trap and is compared to the stretched state to determine deformation. The deformation response of untreated red blood cells and neutrophils were compared to chemically treated cells. Statistically significant differences were observed between normal, diamide-treated, and glutaraldehyde-treated red blood cells, as well as between normal and cytochalasin D-treated neutrophils. Based on the behavior of the pure, untreated populations of red cells and neutrophils, a mixed population of these cells was tested and the discrete populations were identified by deformability.
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影响因子：
6.1
作者：
Cho SH;Chen CH;Tsai FS;Godin JM;Lo YH
通讯作者：
Lo YH
影响因子：
3.8
作者：
Applegate, RW;Squier, J;Marr, DWM
通讯作者：
Marr, DWM
影响因子：
1.7
作者：
Basiji, David A.;Ortyn, William E.;Morrissey, Philip
通讯作者：
Morrissey, Philip
影响因子：
8.6
作者：
Guck, J;Ananthakrishnan, R;Käs, J
通讯作者：
Käs, J
影响因子：
64.8
作者：
通讯作者：
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