Semiconductor nanomembrane tubes: three-dimensional confinement for controlled neurite outgrowth.
Semiconductor nanomembrane tubes: three-dimensional confinement for controlled neurite outgrowth.
复制标题
DOI：
10.1021/nn103618d
复制
发表时间：
2011-04-26
期刊：
影响因子：
17.1
通讯作者：
Williams JC
中科院分区：
文献类型：
--
作者：
Yu M;Huang Y;Ballweg J;Shin H;Huang M;Savage DE;Lagally MG;Dent EW;Blick RH;Williams JC
在许多神经培养研究中，神经突在平坦开放表面上的迁移并不能反映体内三维（3D）微环境。考虑到这一点，我们使用应变硅（Si）和锗（Ge）纳米膜制造了半导体管阵列，并将它们用作初级皮层神经元的细胞培养基质。我们的实验表明，SiGe衬底和管的制造工艺对神经元细胞是生物可行的。我们还观察到，即使在没有粘附因子的情况下，神经元也会被管状结构所吸引，并在生长过程中被引导穿过管状结构。再加上选择性地在靠近管开口的地方播种单个神经元，管内的生长可以限制在单个轴突上。此外，管状结构在物理上和电学上都与天然髓磷脂相似，可以控制管状结构的直径接近轴突的直径，从而与轴突膜形成有限的三维接触，并有可能使其与细胞外溶液绝缘。
In many neural culture studies, neurite migration on a flat, open surface does not reflect the three-dimensional (3D) microenvironment in vivo. With that in mind, we fabricated arrays of semiconductor tubes using strained silicon (Si) and germanium (Ge) nanomembranes and employed them as a cell culture substrate for primary cortical neurons. Our experiments show that the SiGe substrate and the tube fabrication process are biologically viable for neuron cells. We also observe that neurons are attracted by the tube topography, even in the absence of adhesion factors, and can be guided to pass through the tubes during outgrowth. Coupled with selective seeding of individual neurons close to the tube opening, growth within a tube can be limited to a single axon. Furthermore, the tube feature resembles the natural myelin, both physically and electrically, and it is possible to control the tube diameter to be close to that of an axon, providing a confined 3D contact with the axon membrane and potentially insulating it from the extracellular solution.
登录
查看更多内容
影响因子：
5.3
作者：
Hu, Xindao;Viesselmann, Chris;Dent, Erik W.
通讯作者：
Dent, Erik W.
神经袖带电极几何形状对高频神经传导阻滞中起始反应放电的影响。
DOI：
10.1109/tnsre.2010.2071882
发表时间：
2010-12
期刊：
IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society
影响因子：
--
作者：
Ackermann DM Jr;Bhadra N;Foldes EL;Wang XF;Kilgore KL
通讯作者：
Kilgore KL
影响因子：
4
作者：
Mendach, S.;Schumacher, O.;Holz, M.
通讯作者：
Holz, M.
DOI：
10.1049/ip-nbt:20040706
发表时间：
2004-06-01
期刊：
IEE proceedings. Nanobiotechnology
影响因子：
--
作者：
Nam, Y;Chang, J;Wheeler, B C
通讯作者：
Wheeler, B C
影响因子：
14
作者：
Goldner, JS;Bruder, JM;Hoffman-Kim, D
通讯作者：
Hoffman-Kim, D