Expression Analysis of Molecular Chaperones Hsp70 and Hsp90 on Development and Metabolism of Different Organs and Testis in Cattle (Cattle-yak and Yak).
Expression Analysis of Molecular Chaperones Hsp70 and Hsp90 on Development and Metabolism of Different Organs and Testis in Cattle (Cattle-yak and Yak).
复制标题
DOI：
10.3390/metabo12111114
复制
发表时间：
2022-11-15
期刊：
影响因子：
4.1
通讯作者：
中科院分区：
文献类型：
--
作者：
Hsp 70和Hsp 90在睾丸发育和精子发生调控中起重要作用，但Hsp 70和Hsp 90与牛代谢应激的确切关系尚不清楚。本研究以雄性牦牛和牦牛为研究对象，研究了Hsp 70和Hsp 90在不同组织中的表达和定位，并探讨了这些因素对牦牛生长发育和代谢的影响。在我们的研究中，共54头牛（24头牦牛和30头牦牛;年龄1天至10岁）进行了检查。首次克隆了黄牛牦牛Hsp 90 mRNA，并与牦牛Hsp 90 mRNA进行比较，发现其氨基酸序列存在变异，导致蛋白质空间结构存在差异。采用实时荧光定量PCR（RT-qPCR）和Western blot（WB）技术，研究了牦牛和黄牛热休克蛋白70（Hsp 70）和热休克蛋白90（Hsp 90）mRNA和蛋白的表达差异。我们发现，在不同的非生殖器官和睾丸组织中，在不同的发展阶段，Hsp 70和Hsp 90 mRNA和蛋白质表达的差异，而高表达的青少年和成年人的睾丸中观察到。另外，牦牛中Hsp 70的表达量显著高于牦牛，而牦牛-牦牛中Hsp 90的表达量显著高于牦牛（p < 0.01）。免疫组化（IHC）和免疫荧光（IF）技术检测Hsp 70和Hsp 90在睾丸组织中的定位，结果显示Hsp 70和Hsp 90在睾丸上皮细胞、生精细胞和间充质细胞中呈阳性表达。综上所述，我们的研究证明了Hsp 70和Hsp 90在不同组织（肾脏、心脏、小脑、肝脏、肺、脾脏和睾丸）中的表达存在差异，并且Hsp 90在牦牛睾丸中的表达较高，提示牦牛的发育异常可能与Hsp 90的过度代谢有关。
Hsp70 and Hsp90 play an important role in testis development and spermatogenesis regulation, but the exact connection between Hsp70 and Hsp90 and metabolic stress in cattle is unclear. Here, we focused on the male cattle–yak and yak, investigated the expression and localization of Hsp70 and Hsp90 in their tissues, and explored the influence of these factors on development and metabolism. In our study, a total of 54 cattle (24 cattle–yaks and 30 yaks; aged 1 day to 10 years) were examined. The Hsp90 mRNA of the cattle–yak was first cloned and compared with that of the yak, and variation in the amino acid sequence was found, which led to differences in protein spatial structure. Using real-time quantitative PCR (RT-qPCR) and Western blot (WB) techniques, we investigated whether the expression of Hsp70 and Hsp90 mRNA and protein are different in the cattle–yak and yak. We found a disparity in Hsp70 and Hsp90 mRNA and protein expression in different non-reproductive organs and in testicular tissues at different stages of development, while high expression was observed in the testes of both juveniles and adults. Moreover, it was intriguing to observe that Hsp70 expression was significantly high in the yak, whereas Hsp90 was high in the cattle–yak (p < 0.01). We also examined the location of Hsp70 and Hsp90 in the testis by immunohistochemical (IHC) and immunofluorescence (IF) techniques, and the results showed that Hsp70 and Hsp90 were positive in the epithelial cells, spermatogenic cells, and mesenchymal cells. In summary, our study proved that Hsp70 and Hsp90 expressions were different in different tissues (kidney, heart, cerebellum, liver, lung, spleen, and testis), and Hsp90 expression was high in the testis of the cattle–yak, suggesting that dysplasia of the cattle–yak may correlate with an over-metabolism of Hsp90.
登录
查看更多内容
影响因子：
3.2
作者：
通讯作者：
--
影响因子：
2.7
作者：
Abd El-Fattah, Amal Ahmed;Fahim, Atef Tadros;Ali, Bassam Mohamed
通讯作者：
Ali, Bassam Mohamed
DOI：
10.1007/s004210050579
发表时间：
1999-08-01
期刊：
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY AND OCCUPATIONAL PHYSIOLOGY
影响因子：
--
作者：
Paroo, Z;Tiidus, PM;Noble, EG
通讯作者：
Noble, EG
福美双通过干扰肉鸡的心脏代谢来诱导心肌氧化损伤和细胞凋亡。
影响因子：
6.8
作者：
Mo, Quan;Kulyar, Muhammad Fakhar-e-Alam;Li, Jiakui
通讯作者：
Li, Jiakui
影响因子：
2.4
作者：
Motiei, M.;Tavalaee, M.;Nasr-Esfahani, M. -H.
通讯作者：
Nasr-Esfahani, M. -H.