Disparate temperature-dependent virus-host dynamics for SARS-CoV-2 and SARS-CoV in the human respiratory epithelium.
Disparate temperature-dependent virus-host dynamics for SARS-CoV-2 and SARS-CoV in the human respiratory epithelium.
复制标题
人类呼吸道上皮中SARS-CoV-2和SARS-CoV的不同温度依赖病毒宿主动力学。
DOI：
10.1371/journal.pbio.3001158
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发表时间：
2021-03
期刊：
影响因子：
9.8
通讯作者：
Dijkman R
中科院分区：
文献类型：
--
作者：
V'kovski P;Gultom M;Kelly JN;Steiner S;Russeil J;Mangeat B;Cora E;Pezoldt J;Holwerda M;Kratzel A;Laloli L;Wider M;Portmann J;Tran T;Ebert N;Stalder H;Hartmann R;Gardeux V;Alpern D;Deplancke B;Thiel V;Dijkman R
自2019年12月出现以来，严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）已在全球蔓延，成为一项重大公共卫生负担。尽管其与SARS-CoV有密切的系统发育关系，但SARS-CoV-2表现出更强的人际传播动态，可能是由于在感染者上呼吸道上皮中有效的早期复制。由于人类上呼吸道和下呼吸道的不同温度（分别为33°C和37°C）已被证明会影响几种呼吸道病毒的复制动力学以及宿主先天免疫反应动力学，因此我们使用原代人气道上皮细胞培养模型研究了温度对SARS-CoV-2和SARS-CoV感染的影响。与SARS-CoV相比，SARS-CoV-2在33°C而不是37°C下进行感染时复制的滴度更高。尽管这两种病毒都对I型和III型干扰素预处理高度敏感，但详细的时间分辨转录组分析显示，SARS-CoV-2诱导的温度依赖性干扰素和促炎反应与其在33°C或37°C下的复制效率成反比。这些数据为关键病毒-宿主相互作用动力学提供了重要见解，并符合SARS-CoV-2和SARS-CoV的典型临床特征及其各自的传播效率。下呼吸道的温度比上呼吸道高。本研究比较了SARS-CoV-2和SARS-CoV感染期间病毒复制动力学和宿主细胞反应，发现人呼吸道上皮温度影响SARS-CoV-2的病毒复制和病毒-宿主动力学，而对SARS-CoV没有影响。
Since its emergence in December 2019, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has spread globally and become a major public health burden. Despite its close phylogenetic relationship to SARS-CoV, SARS-CoV-2 exhibits increased human-to-human transmission dynamics, likely due to efficient early replication in the upper respiratory epithelium of infected individuals. Since different temperatures encountered in the human upper and lower respiratory tract (33°C and 37°C, respectively) have been shown to affect the replication kinetics of several respiratory viruses, as well as host innate immune response dynamics, we investigated the impact of temperature on SARS-CoV-2 and SARS-CoV infection using the primary human airway epithelial cell culture model. SARS-CoV-2, in contrast to SARS-CoV, replicated to higher titers when infections were performed at 33°C rather than 37°C. Although both viruses were highly sensitive to type I and type III interferon pretreatment, a detailed time-resolved transcriptome analysis revealed temperature-dependent interferon and pro-inflammatory responses induced by SARS-CoV-2 that were inversely proportional to its replication efficiency at 33°C or 37°C. These data provide crucial insight on pivotal virus–host interaction dynamics and are in line with characteristic clinical features of SARS-CoV-2 and SARS-CoV, as well as their respective transmission efficiencies. The temperature in the lower respiratory tract is higher than in the upper respiratory tract. This study compares the viral replication kinetics and host cell response during SARS-CoV-2 and SARS-CoV infection, finding that the temperature of the human respiratory epithelium influences the viral replication and virus-host dynamics of SARS-CoV-2, but not of SARS-CoV.
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影响因子：
64.5
作者：
Hou, Yixuan J.;Okuda, Kenichi;Baric, Ralph S.
通讯作者：
Baric, Ralph S.
影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
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通讯作者：
Huber W
影响因子：
4.7
作者：
Holwerda, Melle;Kelly, Jenna;Dijkman, Ronald
通讯作者：
Dijkman, Ronald
影响因子：
28.3
作者：
Gorbalenya, Alexander E.;Baker, Susan C.;Ziebuhr, John
通讯作者：
Ziebuhr, John