Recent advances in analytical strategies for mass spectrometry-based lipidomics.
Recent advances in analytical strategies for mass spectrometry-based lipidomics.
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基于质谱的脂质组学分析策略的最新进展
DOI：
10.1016/j.aca.2020.09.060
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发表时间：
2020-11-15
影响因子：
6.2
通讯作者：
Xu G
中科院分区：
文献类型：
--
作者：
Xu T;Hu C;Xuan Q;Xu G
脂质是重要的生物分子，在哺乳动物生物体的细胞功能中起着多种作用，如细胞膜锚定、信号转导、物质运输和能量储存。由于脂质的生物学意义，脂质组学已成为组学领域的一门新兴科学。生物系统中的脂质组由数十万个具有复杂结构、多种类别和不同物理化学性质的单个脂质分子组成，这些脂质分子由极性头基和疏水脂肪酰基链的不同组合组装而成。这种结构复杂性对全面的脂质组分析提出了巨大的挑战。由于色谱分离技术的巨大创新和质谱检测工具的不断进步，脂质组学的分析策略已经高度多样化，从而极大地增强了脂质组学的深度和广度。本文将介绍基于质谱的分析策略，包括非靶向，靶向和伪靶向脂质组学的现状。本文综述了近年来脂质组学在生物标志物发现、致病机制和治疗策略等方面的典型应用，并讨论了脂质组学领域面临的挑战。分析技术的最新进展使人们能够快速、可靠和灵敏地检测数百至数千种脂质。基于MS的多样化分析策略极大地促进了脂质组学的发展和应用。多组学技术的整合在研究肿瘤的发病机制和潜在的靶向治疗方面具有广阔的前景。脂质体的综合分析在分析技术上仍面临相当大的挑战。
Lipids are vital biological molecules and play multiple roles in cellular function of mammalian organisms such as cellular membrane anchoring, signal transduction, material trafficking and energy storage. Driven by the biological significance of lipids, lipidomics has become an emerging science in the field of omics. Lipidome in biological systems consists of hundreds of thousands of individual lipid molecules that possess complex structures, multiple categories, and diverse physicochemical properties assembled by different combinations of polar headgroups and hydrophobic fatty acyl chains. Such structural complexity poses a huge challenge for comprehensive lipidome analysis. Thanks to the great innovations in chromatographic separation techniques and the continuous advances in mass spectrometric detection tools, analytical strategies for lipidomics have been highly diversified so that the depth and breadth of lipidomics have been greatly enhanced. This review will present the current state of mass spectrometry-based analytical strategies including untargeted, targeted and pseudotargeted lipidomics. Recent typical applications of lipidomics in biomarker discovery, pathogenic mechanism and therapeutic strategy are summarized, and the challenges facing to the field of lipidomics are also discussed. Recent advances in analytical techniques allow rapid, reliable and sensitive detection of hundreds to thousands of lipids The diversified MS-based analytical strategies have greatly enhanced the development and application of lipidomics. The integration of multi-omics has a bright prospect in studying the pathogenesis and potentially targetable therapy. Comprehensive analysis of the lipidome still faces considerable challenges in analytical techniques.
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影响因子：
6
作者：
Afshinnia F;Rajendiran TM;Karnovsky A;Soni T;Wang X;Xie D;Yang W;Shafi T;Weir MR;He J;Brecklin CS;Rhee EP;Schelling JR;Ojo A;Feldman H;Michailidis G;Pennathur S
通讯作者：
Pennathur S
影响因子：
30.3
作者：
Eisfeld AJ;Halfmann PJ;Wendler JP;Kyle JE;Burnum-Johnson KE;Peralta Z;Maemura T;Walters KB;Watanabe T;Fukuyama S;Yamashita M;Jacobs JM;Kim YM;Casey CP;Stratton KG;Webb-Robertson BM;Gritsenko MA;Monroe ME;Weitz KK;Shukla AK;Tian M;Neumann G;Reed JL;van Bakel H;Metz TO;Smith RD;Waters KM;N'jai A;Sahr F;Kawaoka Y
通讯作者：
Kawaoka Y
影响因子：
16.1
作者：
Butler LM;Perone Y;Dehairs J;Lupien LE;de Laat V;Talebi A;Loda M;Kinlaw WB;Swinnen JV
通讯作者：
Swinnen JV
DOI：
10.1007/s13361-014-1013-x
发表时间：
2015-01-01
影响因子：
3.2
作者：
Almeida, Reinaldo;Pauling, Josch Konstantin;Ejsing, Christer S.
通讯作者：
Ejsing, Christer S.
一种用于大规模检测、鉴定和定量广泛目标代谢物的新型综合方法：在水稻代谢组学研究中的应用。
影响因子：
27.5
作者：
Chen, Wei;Gong, Liang;Luo, Jie
通讯作者：
Luo, Jie