Integrating technologies for comparing 3D gene expression domains in the developing chick limb.
Integrating technologies for comparing 3D gene expression domains in the developing chick limb.
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DOI：
10.1016/j.ydbio.2008.01.031
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发表时间：
2008-05-01
影响因子：
2.7
通讯作者：
Buckland, Richard A.
中科院分区：
文献类型：
--
作者：
Fisher, Malcolm E.;Clelland, Allyson K.;Bain, Andrew;Baldock, Richard A.;Murphy, Paula;Downie, Helen;Tickle, Cheryll;Davidson, Duncan R.;Buckland, Richard A.
由于鸡胚的可获取性和可操作性，它是脊椎动物发育的良好模型。最近来自全基因组测序和EST项目的可用基因组数据的大量增加为鉴定许多新的发育重要的鸡基因提供了机会。传统的记录特定基因在胚胎中表达的时间和地点的方法，使用的是全胚杂交和切片原位杂交，不容易对三维（3D）表达模式进行欣赏，但这可以通过最近开发的显微镜技术，光学投影断层扫描（OPT）来完成。在这里，我们展示了来自不同实验室的发育中的小鸡翅膀的OPT数据可以可靠地集成到一个共同的数据库中，OPT在捕获3D基因表达域方面是有效的，并且可以对这些域进行有意义的比较。新的协议是用来比较三维表达域的7个基因已知参与小鸡翅膀的发展。这揭示了以前未被重视的关系，并展示了利用现代基因组资源建立大规模基因表达3D图谱的潜力。这样的图谱可以扩展到包括其他类型的数据，比如命运图，而且这种方法也更普遍地适用于胚胎、器官和组织。
Chick embryos are good models for vertebrate development due to their accessibility and manipulability. Recent large increases in available genomic data from both whole genome sequencing and EST projects provide opportunities for identifying many new developmentally important chicken genes. Traditional methods of documenting when and where specific genes are expressed in embryos using wholemount and section in-situ hybridisation do not readily allow appreciation of 3-dimensional (3D) patterns of expression, but this can be accomplished by the recently developed microscopy technique, Optical Projection Tomography (OPT). Here we show that OPT data on the developing chick wing from different labs can be reliably integrated into a common database, that OPT is efficient in capturing 3D gene expression domains and that such domains can be meaningfully compared. Novel protocols are used to compare 3D expression domains of 7 genes known to be involved in chick wing development. This reveals previously unappreciated relationships and demonstrates the potential, using modern genomic resources, for building a large scale 3D atlas of gene expression. Such an atlas could be extended to include other types of data, such as fate maps, and the approach is also more generally applicable to embryos, organs and tissues.
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影响因子：
--
作者：
Albertson, RC;Kocher, TD
通讯作者：
Kocher, TD
影响因子：
4.6
作者：
Arques, Carlos G.;Doohan, Roisin;Torres, Miguel
通讯作者：
Torres, Miguel
影响因子：
3
作者：
Baldock, RA;Bard, JBL;Davidson, DR
通讯作者：
Davidson, DR
影响因子：
2.5
作者：
Bell, GW;Yatskievych, TA;Antin, PB
通讯作者：
Antin, PB
影响因子：
2.6
作者：
DEALY, CN;ROTH, A;KOSHER, RA
通讯作者：
KOSHER, RA