Protein composition of the outermost exosporium-like layer of Clostridium difficile 630 spores.
Protein composition of the outermost exosporium-like layer of Clostridium difficile 630 spores.
复制标题
DOI：
10.1016/j.jprot.2015.03.035
复制
发表时间：
2015-06-18
影响因子：
3.3
通讯作者：
Paredes-Sabja D
中科院分区：
文献类型：
--
作者：
Díaz-González F;Milano M;Olguin-Araneda V;Pizarro-Cerda J;Castro-Córdova P;Tzeng SC;Maier CS;Sarker MR;Paredes-Sabja D
艰难梭菌孢子被认为是艰难梭菌感染、传播和持续感染的形态类型。关于艰难梭菌孢子最外层的外孢子层的组成缺乏信息。利用最近发展起来的外孢子菌去除方法，结合MS/MS，我们建立了一种分析艰难梭菌630菌株外孢子体蛋白质组的无胶方法。艰难梭菌孢子外孢子层共发现184种蛋白质。我们鉴定了7个特征性的孢子壳和/或外孢子体蛋白；6个可能参与孢子抗性的蛋白；6个可能参与致病的蛋白；13个未知的蛋白；以及146个可能在组装过程中被包裹到外孢子体中的胞质蛋白，与炭疽芽孢杆菌和蜡状芽孢杆菌的报道类似。通过FLAG融合，我们发现CotA和CotB主要位于孢子被层，而外孢子型胶原糖蛋白(即BclA1、BclA2和BclA3)、外孢子体形态发生蛋白CDEC和CdeM以及未鉴定的外孢子体蛋白CdeA和CDEB主要位于艰难梭菌630孢子的外孢子层。这项研究提供了艰难梭菌外孢子菌蛋白的新候选者，作为检测、清除和基于孢子的治疗的合适靶标。
Clostridium difficile spores are considered the morphotype of infection, transmission and persistence of C. difficile infections. There is a lack of information on the composition of the outermost exosporium layer of C. difficile spores. Using recently developed exosporium removal methods combined with MS/MS, we have established a gel-free approach to analyze the proteome of the exosporium of C. difficile spores of strain 630. A total of 184 proteins were found in the exosporium layer of C. difficile spores. We identified 7 characterized spore coat and/or exosporium proteins; 6 proteins likely to be involved in spore resistance; 6 proteins possibly involved in pathogenicity; 13 uncharacterized proteins; and 146 cytosolic proteins that might have been encased into the exosporium during assembly, similarly as reported for Bacillus anthracis and Bacillus cereus spores. We demonstrate through Flag-fusions that CotA and CotB are mainly located in the spore coat, while the exosporium collagen-like glycoproteins (i.e. BclA1, BclA2 and BclA3), the exosporium morphogenetic proteins CdeC and CdeM, and the uncharacterized exosporium proteins CdeA and CdeB are mainly located in the exosporium layer of C. difficile 630 spores. This study offers novel candidates of C. difficile exosporium proteins as suitable targets for detection, removal and spore-based therapies.
登录
查看更多内容
影响因子：
4.4
作者：
DelVecchio, Vito G.;Connolly, Joseph P.;Mujer, Cesar V.
通讯作者：
Mujer, Cesar V.
影响因子：
3.2
作者：
Lehmann, Y;Meile, L;Teuber, M
通讯作者：
Teuber, M
影响因子：
--
作者：
Bouillaut, Laurent;McBride, Shonna M;Sorg, Joseph A
通讯作者：
Sorg, Joseph A
影响因子：
2.2
作者：
Escobar-Cortes, Karina;Barra-Carrasco, Jonathan;Paredes-Sabja, Daniel
通讯作者：
Paredes-Sabja, Daniel
影响因子：
3.1
作者：
Deakin, Laura J.;Clare, Simon;Lawley, Trevor D.
通讯作者：
Lawley, Trevor D.