The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
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人ARP2/3复合物由进化保守的亚基组成，并位于动态肌动蛋白丝组装的细胞区域。
DOI：
10.1083/jcb.138.2.375
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发表时间：
1997-07-28
影响因子：
7.8
通讯作者：
Mitchison, TJ
中科院分区：
文献类型：
--
作者：
Welch, MD;DePace, AH;Verma, S;Iwamatsu, A;Mitchison, TJ
Arp2/3蛋白复合物与细胞中肌动蛋白聚合的控制有关。人类复合物由7个亚基组成，其中包括肌动蛋白相关蛋白Arp2和Arp3，以及其他5个亚基，分别是p41-Arc、p34-Arc、p21-Arc、p20-Arc和p16-Arc （Arp复合物）。我们已经确定了所有七个亚基的预测氨基酸序列。每一个在不同的真核生物中都有同源物，这意味着该复合体的结构和功能在进化中是保守的。人类的Arp2和Arp3与其他物种的家族成员非常相似。p41-Arc是WD（色氨酸和天冬氨酸）重复序列蛋白Sop2家族的新成员，可能被翻译后修饰，这表明它可能参与调节复合物的活性和/或定位。p34-Arc, p21-Arc， p20-Arc和p16-Arc定义了新的蛋白家族。我们试图通过确定其细胞内分布来评估Arp2/3复合物在细胞中的功能。Arp3、p34-Arc和p21-Arc定位于静止和运动成纤维细胞的板足，以及单核增生李斯特菌组装的肌动蛋白尾部。在肌动蛋白丝的细胞束中未检测到它们。结合Arp2/3复合体诱导肌动蛋白聚合的能力，这些观察结果表明，该复合体促进肌动蛋白在板足中的组装，并可能参与板足突出。
The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells. The human complex consists of seven subunits which include the actin related proteins Arp2 and Arp3, and five others referred to as p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc (Arp complex). We have determined the predicted amino acid sequence of all seven subunits. Each has homologues in diverse eukaryotes, implying that the structure and function of the complex has been conserved through evolution. Human Arp2 and Arp3 are very similar to family members from other species. p41-Arc is a new member of the Sop2 family of WD (tryptophan and aspartate) repeat–containing proteins and may be posttranslationally modified, suggesting that it may be involved in regulating the activity and/or localization of the complex. p34-Arc, p21-Arc, p20-Arc, and p16-Arc define novel protein families. We sought to evaluate the function of the Arp2/3 complex in cells by determining its intracellular distribution. Arp3, p34-Arc, and p21-Arc were localized to the lamellipodia of stationary and locomoting fibroblasts, as well to Listeria monocytogenes assembled actin tails. They were not detected in cellular bundles of actin filaments. Taken together with the ability of the Arp2/3 complex to induce actin polymerization, these observations suggest that the complex promotes actin assembly in lamellipodia and may participate in lamellipodial protrusion.
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影响因子：
7.8
作者：
Mullins, RD;Stafford, WF;Pollard, TD
通讯作者：
Pollard, TD
DOI：
10.1083/jcb.131.2.385
发表时间：
1995-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kelleher JF;Atkinson SJ;Pollard TD
通讯作者：
Pollard TD
影响因子：
7.5
作者：
COSSART, P
通讯作者：
COSSART, P
影响因子：
4.4
作者：
Couch, FJ;Rommens, JM;Simard, J
通讯作者：
Simard, J
影响因子：
7.5
作者：
Frankel, S;Mooseker, MS
通讯作者：
Mooseker, MS