Testosterone improved erectile function by upregulating transcriptional expression of growth factors in late androgen replacement therapy model rats
Testosterone improved erectile function by upregulating transcriptional expression of growth factors in late androgen replacement therapy model rats
复制标题
睾酮通过上调晚期雄激素替代治疗模型大鼠生长因子的转录表达来改善勃起功能
DOI：
10.1038/s41443-022-00627-8
复制
发表时间：
2022
影响因子：
2.6
通讯作者：
Kimura Kazunori
中科院分区：
文献类型：
--
作者：
Kataoka Tomoya;Ito Hiroto;Mori Taiki;Hotta Yuji;Sanagawa Akimasa;Maeda Yasuhiro;Furukawa-Hibi Yoko;Kimura Kazunori
我们以前的研究表明，大鼠去势后勃起功能随着时间的推移而降低;当去势后4周开始睾酮替代治疗时，勃起功能得到改善。在这项研究中，我们研究了睾酮替代治疗后大鼠勃起功能改善的机制。将30只12周龄大鼠分为去势组（Cast）、去势+皮下注射睾酮组（Cast + T）和假手术组（Sham）。通过使用标准方法评估大鼠的勃起功能以及mRNA和蛋白质表达。为了评估勃起功能，我们测量了海绵体内压、平均动脉压、内皮生长因子的mRNA表达和内皮型一氧化氮合酶（eNOS）的蛋白表达。管型组海绵体内压/平均动脉压比值显著降低，睾酮给药显著改善（P= 0.017）。与Cast组相比，Cast+T组血管内皮生长因子A（VEGF-A）、细胞间粘附分子1（ICAM-1）、转化生长因子β（TGF-β）、神经生长因子（NGF）、α-平滑肌肌动蛋白（α-SMA）、小窝相关蛋白1（Cavin-1）、Cavin-2、Cavin-3、sirtuin 1（Sirt-1）、1-磷酸鞘氨醇1（S1 P1）、S1 P2和S1 P3以及eNOS蛋白表达。替吉奥替代治疗通过增加生长因子和eNOS蛋白来改善去势大鼠的勃起功能。
We previously showed that castration of rats reduced erectile function over time; when testosterone replacement therapy was started 4 weeks after castration, erectile function improved. In this study, we examined the mechanism of improvement in erectile function following testosterone replacement therapy in rats. Thirty 12-week-old rats were divided into castrated (Cast), castrated with subcutaneous administration of testosterone (Cast + T), and sham (Sham) groups. Erectile function and mRNA and protein expression were evaluated in the rats by using standard methods. To assess erectile function, we measured the intracavernosal pressure, mean arterial pressure, mRNA expression of endothelial growth factors, and protein expression of endothelial nitric oxide synthase (eNOS). The intracavernosal pressure/mean arterial pressure ratio was significantly lower in the Cast group, and testosterone administration significantly improved (P= 0.017). Compared to the Cast group, the Cast+T group exhibited significantly increased mRNA expressions of vascular endothelial growth factor A (VEGF-A), intercellular adhesion molecule 1 (ICAM-1), transforming growth factor-β (TGF-β), nerve growth factor (NGF), α-smooth muscle actin (α-SMA), caveolae associated protein 1 (Cavin-1), Cavin-2, Cavin-3, sirtuin 1 (Sirt-1), sphingosine-1-phosphate 1 (S1P1), S1P2, and S1P3 and eNOS protein expression. Testosterone replacement therapy improved erectile function in castrated rats by increasing growth factors and eNOS protein.
登录
查看更多内容
影响因子：
2.6
作者：
Kataoka T;Hotta Y;Yamamoto Y;Fukamoto A;Takeuchi M;Maeda Y;Kimura K
通讯作者：
Kimura K
影响因子：
29
作者：
Liu L;Brown D;McKee M;Lebrasseur NK;Yang D;Albrecht KH;Ravid K;Pilch PF
通讯作者：
Pilch PF
内皮一氧化氮合酶的 S-亚硝基化影响勃起功能
影响因子：
2.6
作者：
P. Kavoussi;Ryan P. Smith;Janine L Oliver;R. Costabile;W. Steers;K. Brown;Kimberly Ronde;J. Lysiak;L. Palmer
通讯作者：
L. Palmer
DOI：
10.1073/pnas.1934494100
发表时间：
2003-09-16
影响因子：
11.1
作者：
Igarashi, J;Erwin, PA;Michel, T
通讯作者：
Michel, T
影响因子：
--
作者：
A. Traish;P. Toselli;Seong-Joo Jeong;Noel N Kim
通讯作者：
A. Traish;P. Toselli;Seong-Joo Jeong;Noel N Kim